高通量测序技术分析榨菜腌制过程中表面白膜的微生物群落结构  

Analysis of the Microbial Community Structure of White Biofilm on Zhacai’s Surface during Pickling Process Using High Throughput Sequencing Technology

作　　者：杨吉霞[1] 曾祥平 李凤珠 贺稚非[1] Yang Jixia;Zeng Xiangping;Li Fengzhu;He Zhifei(College of Food Science,Southwest University,Chongqing 400716;Chongqing Academy of Metrology and Quality Inspection of First Branch,Chongqing 402260)

机构地区：[1]西南大学食品科学学院,重庆400716 [2]重庆市计量质量检测研究院第一分院,重庆402260

出　　处：《中国食品学报》2022年第4期330-337,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金青年科学基金项目(31801529);重庆市基础科学与前沿技术研究项目(cstc2017jcyjAX0249);四川省教育厅川菜发展研究中心科研项目(CC21Z30)。

摘　　要：目的:在榨菜腌制过程中,一种常见的现象是其表面形成白膜。解析白膜的微生物群落结构,识别和防控污染微生物,提高榨菜品质的安全性。方法:分别用PacBio SMRT和Illumina MiSeq对榨菜表面白膜的细菌16S rRNA基因和真菌ITS1基因测序,分析细菌和真菌群落结构。结果:白膜中存在较多种类的细菌和真菌,鉴定细菌的属主要包括:代尔夫特菌、丛毛单胞菌、寡养单胞菌、短波单胞菌,真菌的属主要包括:曲霉、枝孢菌、德巴利酵母、青霉、威克汉姆酵母、哈萨克斯坦酵母。结论:研究结果可为榨菜中微生物的预防、控制提供参考。Objective: A common phenomenon in Zhacai during pickling process was the occurrence of white biofilm on Zhacai’s surface. Currently, little knowledge was obtained about the microorganisms of white biofilm. This study aimed to analyze and elucidate its microbial community structure, providing reference data for identifying and controlling harmful microorganisms and improving the safety of Zhacai. Methods: The bacterial 16 S rRNA gene and fungal ITS1 gene of white biofilm samples were sequenced by PacBio SMRT and Illumina MiSeq sequencing platform respectively. Results:There were multiple genera of bacteria and fungi present in the white biofilm samples. The main genera of bacteria identified included Delftia, Comamonas, Stenorophomonas and Brevundimonas, while the main genera of fungi included Aspergillus, Cladosporium, Debaryomyces, Penicillium, Wickerhamomyces and Kazachstania. Conclusions: The results of this study could provide reference for the prevention and control of microorganisms in Zhacai.

关 键 词：高通量测序 榨菜 生物膜 微生物群落结构 

分 类 号：TS255.53[轻工技术与工程—农产品加工及贮藏工程]