羧甲基壳聚糖复合水凝胶的制备及其性能研究  

Preparation and performance study of carboxymethyl chitosan composite hydrogels

作　　者：李明[1] 刘杨 龚浩 刘迪 任莉 蔡超[1,2] 于广利 LI Ming;LIU Yang;GONG Hao;LIU Di;REN Li;CAI Chao;YU Guang-li(Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China;CP Pharmaceutical Qingdao Co.,Ltd.,Qingdao 266555,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,山东省糖科学与糖工程重点实验室,医药学院,山东青岛266003 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266237 [3]正大制药(青岛)有限公司,山东青岛266555

出　　处：《中国海洋药物》2022年第2期19-27,共9页Chinese Journal of Marine Drugs

基　　金：国家重大新药创制项目(2018ZX09735004);国家自然科学基金重大项目(81991522)资助。

摘　　要：目的 制备羧甲基壳聚糖复合水凝胶并对其黏附和抑菌等性能进行研究,为伤口敷料的开发奠定基础。方法 以羧甲基壳聚糖(CMCS)、氧化淀粉(OST)和单宁酸(TA)为原料,通过席夫碱反应制备系列水凝胶,在对其形貌、流变学、溶胀度和黏附性等性能表征基础上,进一步对其生物相容性和抑菌活性进行评价。结果 CMCS与OST/TA溶液体积比为2∶1时,制备的水凝胶COT-2凝胶化时间最短(8.3±0.9) s,黏附强度最高(13.1 kPa),孔径大小适中(200μm),有可自愈合能力,且对HaCaT细胞没有明显毒性,对金黄色葡萄球菌和大肠杆菌具有良好的抑制作用。结论 水凝胶COT-2具有较强的黏附性、机械性能和可自愈合能力,并且表现出良好的生物相容性和抑菌活性,有开发成为伤口敷料的潜力。Objective Carboxymethyl chitosan composite hydrogels were prepared, and a study on their adhesion, antibacterial activity and other properties was conducted, which laid a foundation for the development and application as wound dressings. Methods A series of hydrogels based on carboxymethyl chitosan(CMCS), oxidized starch(OST) and tannic acid(TA), were prepared through Schiff base reaction. Subsequently, the morphology, rheology, swelling degree and adhesion of hydrogels were characterized and compared as well. In addition, the biocompatibility and antibacterial activity of the hydrogels were also evaluated. Results While the ratio of CMCS solution to OST/TA solution in volume was 2∶1, the prepared hydrogel COT-2 showed excellent properties including the shortest gelation time(8.3 ± 0.9) s, the highest adhesion strength(13.1 kPa), moderate pore size(200 μm), and potential self-healing ability. In addition, the experimental results showed that hydrogel COT-2 had no obvious cytotoxicity to HaCaT cells with good inhibitory effects on Staphylococcus aureus and Escherichia coli. Conclusion Hydrogel COT-2, which possessed good biocompatibility and antibacterial activity with strong adhesion, mechanical property and self-healing ability, could be a potential wound dressing.

关 键 词：羧甲基壳聚糖 水凝胶 生物相容性 抑菌活性 

分 类 号：R943[医药卫生—药剂学]