流行性脑脊髓膜炎的实验室分子诊断研究进展  

Progress of research on molecular diagnosis for meningococcal meningitis

作　　者：石刚 徐颖华[1] 叶强[1] SHI Gang;XU Yinghua;YE Qiang(NHC Key Laboratory of Biotechnology Product Verification Methods and Standardization,China Institute for Food and Drug Control,Beijing,China,102629)

机构地区：[1]中国食品药品检定研究院,国家卫生健康委员会生物技术产品检定方法及其标准化重点实验室,北京102629

出　　处：《分子诊断与治疗杂志》2022年第4期539-542,547,共5页Journal of Molecular Diagnostics and Therapy

基　　金：国家重点研发计划项目(2018YFC1603900);国家科技重大专项(2018ZX10102⁃001)。

摘　　要：流行性脑脊髓膜炎(简称流脑)是由脑膜炎奈瑟菌(Nm)引起的一种急性呼吸道传染病。快速、准确、灵敏的实验室检测方法可为流脑临床诊断和流行病学监测研究提供可靠的手段和依据。随着生物技术的发展,基于不同技术原理的快速检测方法被开发并应用于流脑实验室检测,显著提高了不同层级实验室Nm检测率和灵敏度,减少了流脑早期诊断所需的时间。本文就目前国内外流脑实验室快速和分子诊断的研究进展作一综述,以期为后续流脑的预防和控制奠定一定基础。Meningococcal meningitis is an acute respiratory infectious disease caused by Neisseria meningitidis(Nm).Rapid,accurate and sensitive laboratory testing methods can provide reliable means and basis for clinical diagnosis and epidemiological monitoring of meningitis.With the development of biotechnology,rapid detection methods based on different technical principles have been developed and applied to the laboratory detection of meningitis,which has significantly improved the rate and sensitivity of detection for Nm in laboratories at different levels and reduced the time required for early diagnosis of meningitis.This article reviews the current research progress in rapid and molecular diagnosis of meningococcal meningitis,to lay a certain foundation for the follow⁃up prevention and control of meningitis.

关 键 词：流行性脑脊髓膜炎 Real⁃timePCR LAMP 线性探针技术 全基因组测序 

分 类 号：R744.3[医药卫生—神经病学与精神病学]