4-1BB、CD8在口腔扁平苔藓组织中的表达研究  

Correlation between 4-1BB and CD8 expression in oral lichen planus

作　　者：张雪莲 管翠强 金恩龙[2] ZHANG Xuelian;GUAN Cuiqiang;JIN Enlong(School and Hospital of Stomatology,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学口腔医学院·口腔医院,山西太原030001 [2]山西医科大学第一医院口腔科,山西太原030001

出　　处：《口腔医学》2022年第4期296-299,353,共5页Stomatology

基　　金：山西省重点研发计划(201903D321116)。

摘　　要：目的检测共刺激信号4-1BB及CD8在口腔扁平苔藓(oral lichen planus,OLP)组织中的表达,探讨OLP病损中4-1BB介导CD8;T细胞免疫应答的潜在机制。方法选取31例OLP患者(糜烂型15例、非糜烂型16例),15例健康成人(对照组)为研究对象,收集其临床及病理资料,采用免疫组织化学法检测局部组织中4-1BB、CD8的表达情况。结果OLP患者局部组织中4-1BB及CD8的表达高于对照组(P<0.05),且两者表达呈正相关(r=0.389,P<0.05),糜烂型OLP患者中CD8、4-1BB表达高于非糜烂型(P<0.05)。在OLP不同的临床亚组中,VAS评分、REU评分及基底细胞损伤程度均表现出差异(P<0.05)。Spearman相关性分析结果显示,CD8与基底细胞损伤程度呈正比(P<0.05),CD8、4-1BB分别与VAS评分、REU评分存在正相关性(P<0.05)。结论OLP患者局部组织中4-1BB表达升高,其与疾病活动进展程度呈正相关,4-1BB可能通过调控CD8;T细胞参与OLP的发生发展。Objective To examine the expression of 4-1 BB and CD8 in oral lichen planus(OLP)tissues and to investigate the potential mechanism of 4-1 BB on CD8;T cell-mediated immune response in OLP.Methods Thirty-one OLP cases(15 erosive and 16 non-erosive)and 15 healthy adults(control group)were selected as study subjects and their clinical pathological data were collected.Immunohistochemistry was used to detect the expression of 4-1 BB and CD8.Results The expression of 4-1 BB and CD8 in local tissues of OLP were higher than those in the control group(P<0.05),and the expression of 4-1 BB and CD8 in OLP were positively correlated(r=0.389,P<0.05).Besides,the expression of 4-1 BB and CD8 in erosive OLP were higher than those in non-erosive OLP(P<0.05).There was a difference between OLP subgroups in terms of VAS score,REU score and the degree of basal cell injury(P<0.05).Spearman correlation analysis showed that CD8 was positively correlated with the degree of basal cell injury(P<0.05),and CD8 and 4-1 BB were positively correlated with VAS score and REU score(P<0.05).Conclusion There is an abnormal increase of 4-1 BB in OLP,and the increase is positively correlated with disease activity progression,suggesting that 4-1 BB may be involved in the development of OLP by regulating CD8;T cells.

关 键 词：口腔扁平苔藓 CD8 T细胞 4-1BB REU评分 

分 类 号：R781.5[医药卫生—口腔医学]