陕北3个枣品种果实代谢产物及代谢途径分析  

Analysis of Metabolites and Metabolic Pathways of Three Jujube Cultivars from Northern Shaanxi

作　　者：贾宇尧 王超[1] 石倩倩 韩刚[1] JIA Yuyao;WANG Chao;SHI Qianqian;HAN Gang(College of Forestry,Northwest of A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《食品科学》2022年第10期142-149,共8页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2019YFD1001605);西北农林科技大学科技成果推广重点项目(TGZX2021-27)

摘　　要：为了解陕北3个主栽枣品种(清涧木枣、佳县油枣和延川狗头枣)果实的代谢物质差异,以期为枣果品质分析及功能研究提供参考。采用超高效液相色谱-串联质谱的广泛靶向代谢组学技术结合多元统计分析,测定并比较3个枣品种果实的代谢物。结果表明,3个枣品种中共鉴定出15类462种代谢物,各品种间代谢物的含量差异显著。佳县油枣相比清涧木枣存在36种差异代谢物,而延川狗头枣相比清涧木枣、佳县油枣分别存在193、204种差异代谢物。差异代谢物KEGG通路富集分析发现,氨基酸代谢途径与苯丙素类生物合成途径在延川狗头枣中更活跃,但类黄酮生物合成途径则在清涧木枣、佳县油枣中更旺盛,从而使延川狗头枣积累更多的色氨酸、苯丙氨酸、酪氨酸、组氨酸、缬氨酸、苏氨酸、亮氨酸、异亮氨酸、芥子酸、对香豆酸、肉桂酸及紫丁香苷等氨基酸及酚酸类物质,而清涧木枣和佳县油枣则积累了更多的杨梅苷、黄芪苷、香橙素、柚皮素查耳酮及紫铆素等黄酮类代谢物。从代谢层面发现清涧木枣与佳县油枣具有更近的亲缘关系,而与延川狗头枣关系更远;清涧木枣与佳县油枣药用价值更高,而延川狗头枣营养与口味更突出。This study aimed to understand the difference in metabolites of three major jujube cultivars from north Shaanxi,in order to provide a reference for quality analysis and functional evaluation of jujube fruit.The metabolites in Qingjian Muzao,Jiaxian Youzao and Yanchuan Goutouzao were determined and compared by wildly targeted metabonomics based on ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and multivariate statistical analysis.A total of 462 metabolites,belonging to 15 categories,were identified across the three cultivars,and a significant difference in the contents of metabolites was observed among them.Totally 36 differential metabolites between Jiaxian Youzao and Qingjian muzao were identified.In addition,193 and 204 differential metabolites were identified in Yanchuan Goutouzao compared with qingjian Muzao and Jiaxian Youzao,respectively.KEGG pathway enrichment analysis of the differential metabolites indicated that amino acid metabolism and phenylpropanoid biosynthesis pathway were more active in Yanchuan Goutouzao,while flavonoid biosynthesis pathway was more active in Qingjian Muzao and Jiaxian Youzao.Amino acids such as tryptophan,phenylalanine,tyrosine,histidine,valine,threonine,leucine,isoleucine,sinapic acid,p-coumaric acid and cinnamic acid,as well as phenolic acids such as syringin were accumulated in larger quantities in Yanchuan Goutouzao,while flavonoids such as myricitrin,astragalin,aromadendrine,4,2’,4’,6’-tetrahydroxychalcone and butin were accumulated in larger quantities in Qingjian Muzao and Jiaxian Youzao.At the metabolic level,it was found that qingjianmuzao had a closer genetic relationship with Jiaxian Youzao,but further relationship with Yanchuan Goutouzao.Qingjianmuzao and Jiaxian Youzao had higher medicinal value,while Yanchaun Goutouzao had more prominent nutritional quality and taste.

关 键 词：陕北 枣 品种 超高效液相色谱-串联质谱 代谢组 

分 类 号：S665.1[农业科学—果树学]