干酪乳杆菌SB27降低N-亚硝胺对IEC-6细胞毒性作用研究  

Effect of Lactobacillus casei SB27 on reducing the cytotoxicity of N-nitrosamine to IEC-6 cells

作　　者：王淑梅 邸维 于佳睿 王雪 单艺 易华西[3] WANG Shumei;DI Wei;YU Jiarui;WANG Xue;SHAN Yi;YI Huaxi(College of Food Engineering,Harbin University,Harbin 150086,China;School of Food and Bioengineering,Guangdong Polytechnic of Science and Trade,Guangzhou 510430,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]哈尔滨学院食品工程学院,黑龙江哈尔滨150086 [2]广东科贸职业学院食品与生物工程学院,广东广州510430 [3]中国海洋大学食品科学与工程学院,山东青岛266100

出　　处：《中国酿造》2022年第4期54-58,共5页China Brewing

基　　金：黑龙江省博士后科研启动基金(LBH-Q21107);国家自然科学基金(31801516,32172180);广东省青年创新人才项目(2019GKQNCX042);哈尔滨学院青年博士科研启动基金(HUDF2021113)。

摘　　要：该研究选用具有益生功能的干酪乳杆菌(Lactobacillus casei)SB27为研究对象,采用菌落计数法分析N-亚硝胺(N-二甲基亚硝胺(NDMA)、N-二乙基亚硝胺(NDEA)、N-亚硝基吡咯烷(NPYR)和N-亚硝基哌啶(NPIP)(0~80μg/mL)在MRS液体培养基中对干酪乳杆菌SB27生长的影响,并以维生素C(VC)(50μmol/L)为阳性对照,采用细胞计数试剂盒(CCK)-8法分析干酪乳杆菌SB27(103~10^(6) CFU/mL)降低N-亚硝胺对大鼠肠道黏膜细胞IEC-6毒性的损伤。结果表明,N-亚硝胺在MRS液体培养基中对干酪乳杆菌SB27的生长增殖无显著影响(P>0.05)。干酪乳杆菌SB27可降低NDMA、NDEA、NPYR及NPIP对大鼠肠道黏膜细胞IEC-6的毒性损伤。Using Lactobacillus casei SB27 with probiotic function as research object,the effects of N-nitrosamine(N-dimethyl nitrosamine(NDMA),N-diethyl nitrosamine(NDEA),N-nitropyrrolidine(NPYR)and N-nitropiperidine(NPIP))(0-80μg/ml)on the growth of L.casei SB27 in MRS liquid medium were analyzed by colony counting method.Using vitamin(VC)(50μmol/L)as positive control,the effect of L.casei SB27(103-10^(6) CFU/ml)on reducing the damage of intestinal mucosal IEC-6 cells induced by N-nitrosamine was analyzed by cell counting kit(CCK)-8 method.The results showed that N-nitrosamine had no significant effect on the growth and proliferation of L.casei SB27 in MRS liquid medium(P>0.05).L.casei SB27 could reduce the toxic damage of NDMA,NDEA,NPYR and NPIP to IEC-6 cells.

关 键 词：干酪乳杆菌 N-亚硝胺 IEC-6细胞 毒性作用 

分 类 号：Q939.9[生物学—微生物学]