鼠李糖乳杆菌LP216高密度发酵培养基优化  

Optimization of high-density fermentation medium of Lactobacillus rhamnosus LP216

作　　者：李盼盼 张庆芳 刘春莹 迟乃玉[1,2] LI Panpan;ZHANG Qingfang;LIU Chunying;CHI Naiyu(School of Life Science and Technology,Dalian University,Dalian 116622,China;Marine Microbial Engineering Technology Research Center of Liaoning Province,Dalian 116622,China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]辽宁省海洋微生物工程技术研究中心,辽宁大连116622

出　　处：《中国酿造》2022年第4期87-92,共6页China Brewing

基　　金：国家高技术研究发展计划“863”计划项目(2007AA021306);国家重点研发计划(2018YFC0311100)。

摘　　要：为提高鼠李糖乳酸杆菌LP216生产效益,通过单因素试验、Plackett-Burman(PB)试验和Box-Behnken(BB)试验,对菌株LP216发酵培养基进行优化。结果表明,最佳培养基配方为酵母提取物16 g/L、蛋白胨13 g/L、葡萄糖30 g/L、牛肉膏5 g/L、CH_(3)COONa 4 g/L、吐温801.5 g/L、MgSO_(4)·7H_(2)O 0.25 g/L、柠檬酸二胺2.0 g/L、K_(2)HPO_(4)2.0 g/L、MnSO_(4)0.2 g/L。在此优化条件下,菌株LP216活菌数达8.9×10^(9)CFU/mL,是对照组(MRS培养基)活菌数(3.28×10^(9)CFU/mL)的2.71倍。In order to improve the production efficiency of Lactobacillus rhamnosus LP216,the fermentation medium of strain LP216 was optimized by single factor tests,Plackett Burman(PB)tests and Box Behnken(BB)tests.The results showed that the optimum formula of medium was yeast extract 16 g/L,peptone 13 g/L,glucose 30 g/L,beef extract 5 g/L,CH_(3)COONa 4 g/L,Tween 801.5 g/L,MgSO_(4)·7H_(2)O 0.25 g/L,citric diamine 2.0 g/L,K_(2)HPO_(4)2.0 g/L and MnSO_(4)0.2 g/L.Under the optimal conditions,the viable counts of strain LP216 reached 8.9×10^(9)CFU/ml,which was 2.71 times of control group(MRS medium)(3.28×10^(9)CFU/ml).

关 键 词：鼠李糖乳杆菌 培养基 优化 响应面法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]