2017-2020年广东省副溶血弧菌引起的食源性暴发事件流行病学及病原特征分析  

Epidemiological and etiological characteristics of Vibrio parahaemolyticus strains causing foodborne disease outbreaks in Guangdong Province from 2017 to 2020

作　　者：李柏生[1,2] 李振翠 梁骏华 何冬梅[1,2] 柯碧霞 刘美真[1,2] 龙勇 董活波[1,2] Li Baisheng;Li Zhencui;Liang Junhua;He Dongmei;Ke Bixia;Liu Meizhen;Long Yong;Dong Huobo(Institute of Pathogenic Microbiology of Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510000,China;Guangdong Workstation for Emerging Infectious Disease Control and Prevention,Chinese Academy of Medical Sciences,Guangzhou 510000,China)

机构地区：[1]广东省疾病预防控制中心病原微生物检验所,广州510000 [2]广东新发突发传染病防治工作站,广州510000

出　　处：《中华预防医学杂志》2022年第4期443-447,共5页Chinese Journal of Preventive Medicine

基　　金：广东省科技计划项目 (2021B1212030007)。

摘　　要：目的分析2017—2020年广东省副溶血弧菌暴发事件的基本流行病学及病原特征。方法对2017—2020年广东省87起副溶血弧菌暴发事件的基本流行病学特征进行统计分析,对分离株进行血清分型,并运用PFGE进行分子分型。结果广东省副溶血弧菌暴发事件分布在16个地市,44.8%(39/87)和37.9%(33/87)的暴发事件发生在宾馆饭店和学校单位食堂,食品加工存储不当(40.2%,35/87)和生熟不分引起的交叉污染(25.3%,22/87)是引起副溶血弧菌的食源性疾病暴发的主要原因。患者来源菌株主要血清型为O3:K6(87.5%)和O4:KUT(22.5%)。PFGE谱型提示O3:K6型分离株间的相似值为65.5%~100.0%,O4:KUT型分离株的PFGE谱型相似值为66.5%~100.0%。结论2017—2020年广东省副溶血弧菌引起的食源性暴发事件广泛分布,需加强对宾馆、饭店、学校、单位食堂食品正确处理方面的宣传与教育。O3:K6和O4:KUT血清型是暴发事件的主要血清型,流行菌株之间存在遗传多样性。Objective To study the epidemiological and pathogenic characteristics of Vibrio parahaemolyticus isolated from outbreaks cases in Guangdong Province,2017-2020.Methods Epidemiological characteristics of 87 outbreak events caused by Vibrio parahaemolyticus were analyzed.Strains were serotyped,and then analyzed by pulsed-field gel electrophoresis(PFGE).Results The food-borne disease outbreak caused by Vibrio parahaemolyticus was found in 16 cities.44.8%(39/87)and 37.9%(33/87)of the outbreaks occurred in hotels,restaurants and school canteens,respectively.Improper food processing and storage(40.2%,35/87)and cross contamination caused by indiscriminate raw and cooked food(25.3%,22/87)were the main causes of food-borne disease outbreaks of Vibrio parahaemolyticus.The main serotypes of patient derived strains were O3:K6(87.5%)and O4:KUT(22.5%).The similarity value between O3:K6 type isolates was 65.5%-100.0%,and the PFGE pattern similarity value of O4:KUT type isolates was 66.5%-100.0%.Conclusion Outbreaks caused by Vibrio parahaemolyticus are widely distributed in Guangdong province.It is necessary to strengthen the publicity and education on the correct handling of food in hotels,restaurants,schools,and unit canteens.O3:K6 and O4:KUT serotypes are the main serotypes of the outbreak.There is genetic diversity among the epidemic strains.

关 键 词：副溶血弧菌 食源性疾病 暴发 脉冲场凝胶电泳 

分 类 号：R155.3[医药卫生—营养与食品卫生学]