Engineering DNA quadruplexes in DNA nanostructures for biosensor construction  

作　　者：Jingxin Liu Li Yan Shiliang He Junqing Hu 

机构地区：[1]College of Health Science and Environmental Engineering,Shenzhen Technology University,Shenzhen 518118,China [2]Shenzhen Bey Laboratory,Shenzhen 518132,China

出　　处：《Nano Research》2022年第4期3504-3513,共10页纳米研究（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China(Nos.51972055,81803480,and 21902101);the Shenzhen Science and Technology Research Project(No.JCYJ20180508152903208);the Shenzhen Pengcheng Scholar Program,and Shenzhen Bay Laboratory Open Fund(No.SZBL2020090501002).

摘　　要：DNA quadruplexes are nucleic acid conformations comprised of four strands.They are prevalent in human genomes and increasing efforts are being directed toward their engineering.Taking advantage of the programmability of Watson-Crick base-pairing and conjugation methodology of DNA with other molecules,DNA nanostructures of increasing complexity and diversified geometries have been artificially constructed since 1980s.In this review,we investigate the interweaving of natural DNA quadruplexes and artificial DNA nanostructures in the development of the ever-prosperous field of biosensing,highlighting their specific roles in the construction of biosensor,including recognition probe,signal probe,signal amplifier and support platform.Their implementation in various sensing scenes was surveyed.And finally,general conclusion and future perspective are discussed for further developments.

关 键 词：G-QUADRUPLEX I-MOTIF DNA nanotechnology BIOSENSOR 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] TB383[自动化与计算机技术—控制科学与工程]