重组大肠杆菌产腈水合酶的发酵工艺研究  

Study on Fermentation Technology of Nitrile Hydratase Produced by Recombinant Escherichia Coli

作　　者：齐勇 陈森林 芮枝花 沙云冲 姚崇虎 QI Yong;CHEN Sen-lin;RUI Zhi-hua;SHA Yun-chong;YAO Chong-hu(Anhui Ruibang Biotechnology Co.Ltd.,Maanshan 243100,China)

机构地区：[1]安徽瑞邦生物科技有限公司,安徽马鞍山243100

出　　处：《安徽化工》2022年第3期81-84,共4页Anhui Chemical Industry

摘　　要：目前主要通过生物发酵制备腈水合酶催化3-氰基吡啶合成烟酰胺,但在反应过程中,发酵酶活低并且催化过程中极易生成副产物烟酸,因此通过自主构建的产腈水合酶重组大肠杆菌M910001-pMh1229,对其发酵条件进行了系统研究,采用单因素法对发酵过程中培养基进行优化。通过氮源、碳源的筛选,确定最优的碳氮源、诱导剂浓度。优化后的培养基对该菌发酵产腈水合酶酶活可以达到8653 U/mL,OD600达到了210,酶活对比优化前提高了45%。Nicotinamide synthesis is currently mainly through biological fermentation to prepare nitrile hydratase to cata⁃lyze 3-cyanopyridine,but the reaction process is often accompanied by low fermentation enzyme activity and the by-prod⁃uct nicotinic acid is easily generated during the catalytic process.Therefore,the self-constructed nitrile hydration Enzyme recombinant Escherichia coli M910001-pMh1229,its fermentation conditions were systematically studied,and the single factor method was used to optimize the culture medium during the fermentation process.Through the screening of nitrogen source and carbon source,the optimal carbon and nitrogen source and inducer concentration are determined.The optimized medium can reach 8653 U/mL for nitrile hydratase production by the bacteria,OD600 reaches 210,and the enzyme activi⁃ty is increased by 45%compared to before optimization.

关 键 词：烟酰胺 腈水合酶 重组表达 发酵 碳氮源 

分 类 号：TQ926.6[轻工技术与工程—发酵工程] Q939.97[生物学—微生物学]