黑老虎果实不同部位次生代谢成分及其富集差异  

Analysis of the Secondary Metabolites in Kadsura coccinea Fruit and Their Accumulation Difference in Peel, Pulp and Seed Organs

作　　者：高渐飞[1] 周玮[2] 文锡梅 杨艳[3] GAO Jianfei;ZHOU Wei;WEN Ximei;YANG Yan(Guizhou Mountainous Resources Institute,Guizhou Academy of Sciences,Guiyang 550001,China;Guizhou Industry Polytechnic College,Guiyang 550008,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China)

机构地区：[1]贵州科学院,贵州省山地资源研究所,贵州贵阳550001 [2]贵州工业职业技术学院,贵州贵阳550008 [3]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014

出　　处：《食品工业科技》2022年第12期27-35,共9页Science and Technology of Food Industry

基　　金：贵州省科技计划重大专项(黔科合平台人才[2017]5411号);国家重点研发计划课题(2016YFC0502601)。

摘　　要：为推进黑老虎果实开发利用,利用广泛靶向代谢组学技术鉴定其果皮、果肉、种子中代谢物,基于结构配置进行归类分析。结果显示:在黑老虎果实中共鉴定出307个次生代谢物,其中以酚酸(数量占38.8%)和黄酮(27.7%)占主导;果皮含272个、果肉286个、种子201个,3个部位共有180个;种子中酚酸、类黄酮和萜类化合物数量明显低于果肉和果皮,使其次生代谢物多样性也较低;果皮中次生代谢物丰度(81.62×10^(7))远高于果肉(25.61×10^(7))和种子(24.38×10^(7)),主要因黄酮类成分(花青素、黄酮醇、黄酮)的高度富集和生物碱上调;3个部位间显著差异代谢组分主要为酚酸和黄酮类化合物;黑老虎果皮富含类黄酮(槲皮素、儿茶素和矢车菊素类等),种子中木脂素丰度高,3个部位中都含鉴定出结构新颖的三萜化合物,表明其具有较高的利用和研究价值。In order to explore Kadsura coccinea fruit development and utilization,widely-targeted metabolomics was used to identify the compounds in different organs of K. coccinea fruit, according to their structural distribution and categories,the diversity and abundance of the secondary metabolites were analyzed. Results:A total of 307 secondary metabolites were identified in the fruit of K. coccinea, among which phenolic acids(38.8%) and flavonoids(27.7%) were the dominant. In particular, 272 were found in the peel, 286 in the pulp, 201 in the seed, and 180 in all three parts. The amount of phenolic acids, flavonoids, and terpenoidswas significantly lower in seed than in pulp and peel, resulting in a lower diversity of secondary metabolites. The abundance of secondary metabolites in the peel(81.62×10^(7)) was much higher than that in the pulp(25.61×10^(7)) and seed(24.38×10^(7)), mainly due to the high enrichment offlavonoids(quercetins, catechins and cyanidins) and the significant up-regulation of alkaloids. The metabolic components were mainly phenolic acids and flavonoids. The peel of K. coccinea was rich in flavonoids(quercetin, catechins and cyanidins, etc), and the seeds were rich in lignins. All three parts contained triterpenoids with novel structures, indicating that the fruit had significant utilization potential and research value.

关 键 词：黑老虎果实 不同部位 广泛代谢组学 类黄酮 木脂素 三萜 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程] Q946.8[轻工技术与工程—食品科学与工程]