断乳期人源菌群对小鼠生长发育的影响  

Effect of Human Flora during Weaning on Growth and Development of Mice

作　　者：史玉东 刘梦瑶[1,2] 宋晨 李勇枝 卢卫红[1,2] Shi Yudong;Liu Mengyao;Song Chen;Li Yongzhi;Lu Weihong(School of Chemical Engineering and Chemistry,Harbin Institute of Technology,Harbin 150001;Institute of Extreme Environmental Nutrition and Protection,Harbin Institute of Technology,Harbin 150001;R&D Center,Inner Mongolia Mengniu Dairy(Group)Co.Ltd.,Huhhot 011500;Astronaut Research and Training Centre of China,Beijing 100094)

机构地区：[1]哈尔滨工业大学化工与化学学院,哈尔滨150001 [2]哈尔滨工业大学极端环境营养与防护研究所,哈尔滨150001 [3]内蒙古蒙牛乳业(集团)股份有限公司研发中心,呼和浩特011500 [4]中国航天员科研训练中心,北京100094

出　　处：《中国食品学报》2022年第5期120-128,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2017YFC1601900)。

摘　　要：目的:搭建断乳期人源菌群小鼠模型,研究肠道菌群对小鼠生长发育的影响。方法:将断乳期婴儿肠道菌群移植至无菌昆明小鼠中,建立断乳期人源菌群小鼠模型。断乳期人源菌群小鼠和无菌小鼠各9只,饲喂基础日粮至8周龄。测定小鼠体质量、大脑脂肪酸含量以及血清、肝脏中胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)含量,通过HE染色观察小鼠海马CA1区及结肠发育情况。对比两组小鼠指标差异,分析肠道菌群对小鼠大脑、肠道发育及免疫等相关指标的影响。结果:断乳期人源菌群小鼠健康状况良好,能够部分模拟断乳期婴儿肠道菌群;肠道菌群对小鼠大脑神经元细胞、突触以及结肠绒毛发育有显著影响;对小鼠大脑中单不饱和脂肪酸C16∶1(P<0.001)、C18∶1(P<0.01)和多不饱和脂肪酸C18∶3(P<0.05)、C20∶3(P<0.01)、C20∶4(P<0.001)、C22∶5(P<0.05)、C22∶6(P<0.01)含量有显著影响;对小鼠血清、肝脏中IGF-1的含量有显著影响。结论:断乳期人源菌群小鼠模型的建立,为研究断乳期婴儿肠道菌群对宿主生理功能的影响提供了动物模型。Objective:To establish a model of weaning human floral-associated mice and study on the effect of intestinal flora on the grouth and development of mice.Methods:The intestinal flora of weaned infants was transplanted into germ free Kunming mice to establish a model of weaning human floral-associated mice.Weaning mice with human flora and germ free mice(n=9,for each group)were fed with basic diet until 8 weeks old.The body weight,brain fatty acid content,insulin-like growth factor-1(IGF-1)and in-sulin-like growth factor binding protein-3(IGFBP3)contents in serum and liver were measured.The development of hippocampal CA1 area and colon were observed by HE staining.The differences of indexes between the two groups were compared,and the effects of intestinal flora on brain,intestinal development and immunity were analyzed.Results:The weaning human floral-associated mice were in good health and could partially simulate the intestinal flora of weaning infants.Intestinal flora had significant effects on the development of mice brain neuronal cells,synapses and colonic villi.The contents of monounsaturated fatty acids C16∶1(P<0.001),C18∶1(P<0.01)and polyunsaturated fatty acids C18∶3(P<0.05),C20∶3(P<0.01),C20∶4(P<0.001),C22∶5(P<0.05)and C22∶6(P<0.01)in mouse brain were significantly affected.It had a significant effect on the content of IGF-1 in serum and liver of mice.Conclusion:The weaning human floral-associated mice provides an effective animal model for the study of the effect of intestinal flora on host physiological function in weaning infants.

关 键 词：人源菌群小鼠 断乳期 肠道菌群 发育 免疫 

分 类 号：TS201.4[轻工技术与工程—食品科学]