螺旋藻多糖对秀丽隐杆线虫氧化应激及寿命的影响  

Effects of Spirulina Polysaccharides on Oxidative Stress and Life Span of Caenorhabditis elegans

作　　者：王猛 马浩天 关思宇 狄建兵[2] 王愈[2] 李润植 崔红利 Wang Meng;Ma Haotian;Guan Siyu;Di Jianbing;Wang Yu;Li Runzhi;Cui Hongli(Institute of Molecular Agriculture and Bioenergy,College of Agriculture,Shanxi Agricultural University,Taigu 030801,Shanxi;College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,Shanxi)

机构地区：[1]山西农业大学农学院分子农业与生物能源研究所,山西太谷030801 [2]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《中国食品学报》2022年第5期137-146,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31902394);山西农谷建设科研专项(SXNGJSKYZX201906);晋中市重点研发(农业领域)项目(201903D01100013,Y192012)。

摘　　要：机体多种慢性疾病发生归因于自由基反应失衡诱发的氧化应激,螺旋藻多糖(PSPs)作为一种天然抗氧化物质,其抗氧化、抗衰老作用机制尚不明确。本文采用化学方法测定PSPs体外抗氧化活性。以秀丽隐杆线虫为模式动物,检测PSPs对线虫氧化应激抵抗作用。结果表明:PSPs具有DPPH·清除能力和提升总还原力的作用,其作用效果呈剂量依赖性;PSPs呈剂量依赖性地延长秀丽隐杆线虫寿命;PSPs通过提高运动能力和抗氧化酶SOD、CAT活性,降低衰老色素的积累和细胞内活性氧(ROS)水平来增强抗氧化能力;在1‰H_(2)O_(2)氧化应激压力下,PSPs显著延长线虫寿命。qRT-PCR显示,PSPs上调daf-16、skn-1、sir-2.1等基因mRNA表达量。结论:PSPs通过胰岛素信号通路提高抗氧化酶活性以及相关基因表达来增强氧化应激抵抗能力并延长线虫寿命。s A variety of chronic diseases are attributed to oxidative stress induced by imbalance of free radical reaction.Spirulina polysaccharides(PSPs)are natural active substances with strong antioxidant,but its mechanisms of antioxidant and anti-aging are still unclear.In the current study,the antioxidant activity of PSPs in vitro was determined by chemical methods,and the resistance of PSPs to oxidative stress of Caenorhabditis elegans(C.elegans)was tested in vivo.The results showed that PSPs had DPPH·scavenging capacity and total reducing power promoting capacity,which was dose-dependent.PSPs prolonged the life span of C.elegans in a dose-dependent manner.The antioxidant capacity was enhanced by improving the exercise abilities and the activities of antioxidant enzymes SOD and CAT,reducing the accumulation of senescence pigment and the level of intracellular reactive oxygen species(ROS).Under the oxidative stress of 1‰H_(2)O_(2),PSPs significantly prolonged the life span of C.elegans.The qRT-PCR results indicated that PSPs could significantly up-regulate the mRNA expression of daf-16,skn-1,and sir-2.1 genes.These results suggested that PSPs could enhance the resistance to oxidative stress and prolong the life span of C.elegans by increasing the activity of antioxidant enzymes and the expression of related genes through the insulin signaling pathway.

关 键 词：螺旋藻多糖(PSP) 秀丽隐杆线虫 抗氧化 寿命 胰岛素信号通路 

分 类 号：TS201.4[轻工技术与工程—食品科学]