病死猪无害化处理关键菌株分析  

Analysis of Key Strains in Harmless Treatment of Dead Pigs

作　　者：王吉平 张野 王瑾 苏天明 谢育利 梁芷姮 甘国勇 杨振媚 何铁光 WANG Jiping;ZHANG Ye;WANG Jin;SU Tianming;XIE Yuli;LIANG Zhiheng;GAN Guoyong;YANG Zhenmei;HE Tieguang(Agricultural Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;Guangxi Yipu Testing Technology Co. , Ltd. , Nanning 530007, China)

机构地区：[1]广西壮族自治区农业科学院农业资源与环境研究所,广西南宁530007 [2]广西益谱检测技术有限公司,广西南宁530007

出　　处：《湖南生态科学学报》2022年第3期1-7,共7页Journal of Hunan Ecological Science

基　　金：广西重点研发计划(桂科AB20159006);广西农业科学院基本科研业务专项(桂农科2021YT038、桂农科2020YM111);科技先锋队“强农富民”“六个一”专项行动(桂农科盟202213);南宁市科学研究与技术开发计划项目(20182084);南宁市武鸣区科学与技术开发计划(20190112)。

摘　　要：【目的】探究用生物发酵法无害化处理病死猪过程中的关键微生物,为发酵过程机理解析及菌剂复配优化提供参考依据。【方法】利用二代及三代全长测序技术测定细菌16s rDNA序列,研究微生物群落在不同水平下的相对丰度和群落功能变化,确定病死猪无害化处理过程中的关键微生物种类。【结果】用生物发酵法无害化处理病死猪过程中存在优势微生物种群,且群落功能变化与芽孢杆菌属(Bacillus)相对丰度变化趋势一致。【结论】用生物发酵法无害化处理病死猪过程中关键微生物为芽孢杆菌属细菌,且凝结芽孢杆菌类是其中的主要功能种群。【Objective】The key microorganisms in the process of harmless treatment of dead pigs by biological fermentation were explored,to provide a reference basis for the mechanism of the fermentation process and the optimization of bacterial preparation.【Method】The 16s rDNA sequences of bacteria were analyzed by the Illumina sequencing and Pacbio full-length sequencing platforms to study the changes of relative abundance at different levels and community function of microbial community,and determine the key microbial species in the harmless treatment of dead pigs.【Result】There were dominant microbial populations in the process and the changes of community function were consistent with the changes in relative abundance of Bacillus.【Conclusion】Bacillus is the key microorganism in the harmless process of dead pigs,and B.coagulans are the main functional microbes in degrading dead pigs process.

关 键 词：病死猪 无害化 生物发酵 微生物群落 16s rDNA 

分 类 号：X172[环境科学与工程—环境科学]