微波辅助酸水解褐藻糖胶的研究  

Study on Microwave-Assisted Acid Hydrolysis of Fucoidan

作　　者：冯金华[1] 刘红英[1] 姜帅 郑文静 FENG Jin-hua;LIU Hong-ying;JIANG Shuai(Ocean College of Hebei Agricultural University,Qinhuangdao,Hebei 066003;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306)

机构地区：[1]河北农业大学海洋学院,河北秦皇岛066003 [2]上海海洋大学水产与生命学院,上海201306 [3]青岛科技大学环境与安全工程学院,山东青岛266042

出　　处：《安徽农业科学》2022年第11期154-158,共5页Journal of Anhui Agricultural Sciences

基　　金：河北省高等学校科学技术研究项目(yd2013030);河北省自然科学基金项目:(D2014204127);河北农业大学大学生创新创业训练计划项目(2018048);河北农业大学海洋学院创新创业项目“虾壳中虾青素的皂化条件优化及双水相萃取研究”。

摘　　要：[目的]研究微波辅助酸解在低分子量海带褐藻糖胶制备中的可行性。[方法]采用酸提取法制备海带褐藻糖胶,测定在不同HCl浓度(0、1、2 mol/L)、不同降解时间(40、50、60 min)、不同降解温度(70、80、90℃)条件下微波辅助酸解得到的褐藻糖胶的分子量,分析不同降解条件对褐藻糖胶分子量的影响,并与水浴辅助酸解的结果进行对比分析。[结果]随着HCl浓度、降解时间、降解温度的升高,酸解产物的分子量降低,且温度对降解产物分子量的影响最大。与水浴加热相比,微波辅助酸解降解产物的分子量更低。在80℃,HCl浓度为1 mol/L,降解40 min以上,比较容易制备低分子量褐藻糖胶。微波比水浴加热更容易对褐藻糖胶的硫酸基造成破坏。在2 mol/L HCl条件下,微波和水浴辅助酸解60 min,分别在70和90℃观察到明显的硫酸基脱除现象。[结论]采用微波辅助酸解法制备低分子量褐藻糖胶时,要注意硫酸基脱除的问题。[Objective]To study the feasibility of microwave-assisted acidolysis in the preparation of low molecular weight kelp fucoidan.[Method]Kelp fucoidan was prepared by acid extraction.The molecular weight of fucoidan obtained by microwave-assisted acidolysis under different HCl concentration(0,1,2 mol/L),different degradation time(40,50,60 min)and different degradation temperature(70,80,90℃),was measured.The effects of different degradation conditions on the molecular weight of fucoidan were analyzed and compared with the results of water bath assisted acidolysis.[Result]With the increase of HCl concentration,degradation time and degradation temperature,the molecular weight of acid hydrolysis products decreased,and the temperature had the greatest influence on the molecular weight of degradation products.Compared with water bath heating,the molecular weight of microwave-assisted acid hydrolysis degradation products was lower.It was easy to prepare low molecular weight fucoidan at 80℃,HCl concentration of 1 mol/L and degradation for more than 40 min.Microwave was easier to destroy the sulfate group of fucoidan than water bath heating.Under the condition of 2 mol/L HCl,microwave and water bath assisted acidolysis for 60 min,obvious sulfate removal was observed at 70℃and 90℃respectively.[Conclusion]When preparing low molecular weight fucoidan by microwave-assisted acidolysis,attention should be paid to the removal of sulfate group.

关 键 词：褐藻糖胶 酸解 微波辅助 分子量 

分 类 号：TQ461[化学工程—制药化工]