6-苄基腺嘌呤对大豆内部水分分布及其生长状态的影响  

Effects of 6-Benzylaminopurine on internal water distribution and growth state of soybean

作　　者：顾莹 陈毅 岳霞 程恩思 宋平[1] Gu Ying;Chen Yi;Yue Xia;Cheng Ensi;Song Ping(College of Information and Electrical Engineering,Shenyang Agricultural University,Shenyang 110161,China;College of Engineering,Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]沈阳农业大学信息与电气工程学院,沈阳110161 [2]沈阳农业大学工程学院,沈阳110161

出　　处：《农业工程学报》2022年第5期303-308,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金项目(31701318,31811540396);辽宁省基础研究项目(LSNJC201916)。

摘　　要：为研究大豆发芽过程中加入不同浓度的6-苄基腺嘌呤(6-Benzylaminopurine,6-BA)后大豆内部水分的分布及对其生长状态的影响,以中黄13为研究对象,在相同环境条件下,用浓度分别为0(对照)、1、3、5、7、9 mg/L的6-BA溶液处理大豆。利用低场核磁共振技术(Low Field Nuclear Magnetic Resonance,LF-NMR)对中黄13在浸泡8 h及萌发12、24、36、48、60 h时间点的波谱信息、图像信息进行采集,同时对豆芽的生长状态进行理化指标和形态指标检测。结果显示:与其他处理组相比,浓度为3 mg/L的6-BA溶液处理的大豆的发芽周期、吸水率、平均质量、发芽率、豆芽轴长、轴径各指标表现均为最优;低浓度(1、3 mg/L)6-BA加速大豆蛋白质的水解,导致其渗透势降低,促进大豆蛋白质体吸收更多的水分,进而促使豆芽轴长变长、轴径变粗;高浓度(5、7、9 mg/L)6-BA使大豆细胞壁在细胞的渗透作用下遭到破坏,导致大豆吸水率降低,进而抑制了大豆的生长。该研究从大豆原料的预处理开始对整个加工过程进行了研究,为豆芽的生产加工提供一定的理论支持和数据参考。Soybean sprout is one type of edible vegetable that is cultivated by sprouting soybeans.Taking Zhonghuang 13 as the research object,a series of treatments were carried out using the 6-BA solution with concentrations of 0,1,3,5,7,and 9 mg/L under the same environmental conditions.The water distribution in soybean was measured to determine the growth state under the different concentrations of 6-BA during soybean germination.The low-field nuclear magnetic resonance(LF-NMR)and magnetic resonance imaging(MRI)were selected to clarify the effect of 6-BA on the germination of soybean.Three procedures included the LF-NMR,LF-NMR/MRI imaging,and the acquirement of soybean physical,chemical,and morphological indexes.The proton density(PD)image was extracted from the nuclear magnetic signals collected by the Carr-Purcell-Meiboom-Gill(CPMG)spin echo pulse sequence.The results show that the water evolution was visually determined during soybean germination using the LF-NMR PD images.The soybean germination was divided into the imbibition(0-8 h),protrusion(8-24 h),and germination stage(24-60 h).The germination cycle from the soaking to germination varied with the concentration of 6-BA solution in the treatment groups.The germination time of soybean seeds with the 0,1,and 3 mg/L of 6-BA solution was shorter than that of the rest,where the best was the 3 mg/L concentration.The water content in various phases of soybean was quantitatively characterized by LF-NMR data.According to the relaxation time,the water content of soybean was divided into three kinds:the bound water T_(21),semi bound water T_(22),and free water T_(23).The sub peak was disappeared gradually on the left side of bound water(0.1 ms<T_(21)<10 ms)with the 6-BA concentration higher than 5 mg/L.The low 6-BA concentration accelerated the hydrolysis of soybean protein to reduce the osmotic potential,where the soybean protein body was promoted to absorb more water.By contrast,the high 6-BA concentration destroyed the soybean cell wall,due to the infiltration of soybean

关 键 词：低场核磁共振 水分 大豆 发芽 6-苄基腺嘌呤 

分 类 号：S24[农业科学—农业电气化与自动化]