固定化短乳杆菌催化合成γ-氨基丁酸的研究  

Synthesis ofγ-Aminobutyric Acid by Immobilized Lactobacillus brevis

作　　者：孙丽慧 贺雷雨[2] 陈业文 宫宇晴 SUN Li-hui;HE Lei-yu;CHEN Ye-wen;GONG Yu-qing(School of Ocean Science and Technology,Dalian University of Technology,Panjin 124221,Liaoning,China;School of Life Science and Medicine,Dalian University of Technology,Panjin 124221,Liaoning,China)

机构地区：[1]大连理工大学海洋科学与技术学院,辽宁盘锦124221 [2]大连理工大学生命科学与药学学院,辽宁盘锦124221

出　　处：《食品研究与开发》2022年第11期183-188,共6页Food Research and Development

基　　金：辽宁省自然科学基金面上项目(2019-MS-044)。

摘　　要：以海藻酸钠为载体,包埋法固定化短乳杆菌,催化L-谷氨酸钠合成γ-氨基丁酸,通过正交试验对细胞固定化条件进行优化,并对固定化细胞的酶学性质及操作稳定性进行研究。确定最佳固定化条件为海藻酸钠浓度2.5%、CaCl2浓度3.0%、固定化细胞平均粒径3.2 mm、硬化时间4 h。在此条件下制备的固定化细胞最适酶反应pH值为4.5,最适温度为35℃,且表现出较好的操作稳定性,重复利用至第7次后仍保持53.2%的相对酶活力。Lactobacillus brevis was immobilized on sodium alginate by embedding method to catalyze the synthesis ofγ-aminobutyric acid from sodium L-glutamate.The conditions for cell immobilization were optimized by orthogonal test,and the enzymatic properties and operational stability of the immobilized cells were studied.The conditions for cell immobilization were optimized as 2.5%sodium alginate,3.0%CaCl2,average particle size of 3.2 mm and immobilization for 4 h.The cells immobilized under the above conditions presented the best performance at pH 4.5 and 35℃.The immobilized cells showed well operational stability and maintained 53.2%relative enzyme activity after reused seven times.

关 键 词：Γ-氨基丁酸 短乳杆菌 海藻酸钠 固定化细胞 包埋法 

分 类 号：TQ226.36[化学工程—有机化工] TQ426