1-MCP和SO_(2)保鲜剂处理阳光玫瑰葡萄的转录组学分析  

Transcriptome analysis of Shine Muscat grape treated with 1-MCP and SO_(2) preservatives

作　　者：成果[1] 谢林君[1] 周思泓 李玮[1] 谢太理[1] 周咏梅[1] 张劲[1] CHENG Guo;XIE Lin-jun;ZHOU Si-hong;LIWei;XIE Tai-li;ZHOU Yong-mei;ZHANG Jin(Grape andWine Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西农业科学院葡萄与葡萄酒研究所,广西南宁530007

出　　处：《南方农业学报》2022年第3期641-653,共13页Journal of Southern Agriculture

基　　金：广西重点研发计划项目(桂科AB18221077,桂科AB18294003);国家现代农业产业技术体系广西创新团队建设专项(nycytxgxcxtd-20-03);广西农业科学院科技发展基金项目(桂农科2021JM107)。

摘　　要：【目的】分析2种保鲜剂处理阳光玫瑰葡萄贮藏阶段基因差异表达情况,为从分子生物学角度研究保鲜剂处理对葡萄果实贮藏品质影响的调控机制提供理论参考。【方法】以阳光玫瑰葡萄为试材,采用1-甲基环丙烯(1-MCP)和二氧化硫(SO_(2))保鲜剂分别对果实进行冰温贮藏(-1~1℃),并以不使用保鲜剂的果实为对照,对贮藏1、5、9、13和17周的2种保鲜剂处理及对照的果实分别取样开展转录组学分析,通过实时荧光定量PCR结果证实转录组测序结果的可靠性。【结果】从对照和2种保鲜剂处理的转录组测序结果中共获得371.64 Gb的原始数据,各样品Clean data均达6.03 Gb,GC含量为46.28%~47.53%,Q30≥93.50%,与葡萄参考基因组的匹配率为75.75%~90.23%。1-MCP处理与对照的差异表达基因数在贮藏后13周达最高值,而SO_(2)处理与对照及SO_(2)处理与1-MCP处理的差异表达基因数在贮藏后5周达最高值。贮藏1周和13周时,1-MCP处理与对照之间差异表达基因最多,分别为965和2881个;贮藏5周和9周时,SO_(2)处理与对照之间差异表达基因最多,分别为3698和1628个;贮藏17周时,处理和对照两两比较获得的差异表达基因相差不大。贮藏1~5周果实内部发生细胞组分、分子功能和生物过程等方面的变化较贮藏中后期更为剧烈,且在MYB、AP2/ERF-ERF、NAC、GARP-G2-like、HB-HD-ZIP和WRKY转录因子的调控下,单萜合成相关结构基因表达量在贮藏5周后迅速降低。基于实时荧光定量PCR的所有样本中苯丙烷—类黄酮、类胡萝卜素和单萜合成代谢路径相关基因表达水平检测结果与转录组测序分析结果基本一致。【结论】1-MCP与SO_(2)保鲜剂对阳光玫瑰葡萄贮藏产生不同影响,前者在贮藏过程中抑制果实成熟和衰老作用释放较后者更为平缓,且通过阻断乙烯与受体结合并抑制ETR、EIN3和ERF1/2这3个乙烯信号通路关键基因表达发挥延缓衰老作用。转�【Objective】To analyze the gene differential expression of Shine Muscat grape during storage under two preservation treatments,so as to provide theoretical reference for studies on the regulation mechanism of preservative treatments on stored grape fruit quality from the perspective of molecular biology.【Method】Shine Muscat grapes were used as test material,treated with 1-MCP and SO_(2)preservative agents respectively and then was put in cryogenic storage(-1-1℃).The fruits without any preservative agent were taken as the control.Transcriptome analysis was performed on the fruits treated with each preservative treatment and the control group after 1,5,9,13 and 17 weeks of storage period,respectively.The liability of sequencing was confirmed by qRT-PCR experiment.【Result】A total of 371.64 Gb of raw data was obtained from the transcriptomic sequencing of groups of two preservative treatments and the control.Clean data of each sample reached 6.03 Gb.GC content was 46.28%-47.53%,and Q30 percentage was more than 93.50%,and the matching rate to grape reference genome was 75.75%-90.23%.The number of DEGs between 1-MCP treatment and control reached the highest value at 13 weeks after storage,while the number of differentially expressed genes(DEGs)between SO_(2)treatment and control,SO_(2)treatment and 1-MCP treatment reached the highest value at 5 weeks after storage.At the 1st and 13th weeks of storage.The number of DEGs between 1-MCP treatment and control was 965 and 2881,respectively.The number of DEGs between SO_(2)treatment and control reached the highest at 3698 and 1628,in the 5th and 9th weeks of storage,respectively.At 17th weeks of storage,no significant difference appeared in the number of differential genes obtained by pairwise comparison between treatment groups and the control.The results also showed that the changes of cellular components,molecular functions and biological processes in fruits during 1-5 weeks of storage were much more dramatic than those in the middle and late stages of storage

关 键 词：阳光玫瑰葡萄 保鲜处理 转录组 差异表达基因 

分 类 号：S663.1[农业科学—果树学]