脂肪酸及表面活性剂对广叶绣球菌液体培养的作用研究  

Effects of fatty acids and surfactants on liquid culture of Sparassis latifolia

作　　者：刘晓瑜 马璐[1] 杨驰[1] 肖冬来[1] 江晓凌[1] 应正河[1] 林衍铨[1] Liu Xiaoyu;Ma Lu;Yang Chi;Xiao Donglai;Jiang Xiaoling;Ying Zhenghe;Lin Yanquan(Institute of Edible Fungi,Fujian Academy of Agricultural Sciences,National and Local Joint Engineering Research Center for Breeding&Cultivation of Featured Edible Fungi,Fuzhou,Fujian 350014,China)

机构地区：[1]福建省农业科学院食用菌研究所特色食用菌繁育与栽培国家地方联合工程研究中心,福建福州350014

出　　处：《食药用菌》2022年第3期226-232,共7页Edible and Medicinal Mushrooms

基　　金：福建省农业科学院科技创新团队项目(CXTD2021016-2);福建省种业创新与产业化工程项目(zycxny2021011);福建省属公益类科研院所基本科研专项(2018R1020-4,2020R1035003)。

摘　　要：广叶绣球菌是近年来实现工厂化栽培的一种珍稀食药用菌,液体培养可缩短其制种时间。试验通过测定菌球密度、菌丝生物量等指标,研究脂肪酸、表面活性剂、有机溶剂等外源添加物对绣球菌液体培养的作用,结果表明:加入2 g/L的油酸和体积分数为0.2%的甲醇对菌球直径、菌球密度和菌丝体生物量均有一定促进作用;0.5 g/L橄榄油和0.4 g/L花生油能显著提高菌球直径和菌丝体生物量;添加4 g/L Tween 80能在一定程度上促进菌丝生长,在培养初期(3天)添加能获得较好的促进效果。Sparassis latifolia is a precious edible and medicinal mushroom that has been cultivated in recent years.Liquid culture can shorten the spawn production time.Influence of exogenous additives such as fatty acids,surfactants,organic solvents on liquid culture of Sparassis latifolia was studied by measuring mycelium pellet density,mycelial biomass and other indicators.Result indicated that 2 g/L oleic acid and 0.2%methanol had a certain promoting effect on mycelium pellet diameter,mycelium pellet density and mycelial biomass;0.5 g/L olive oil and 0.4 g/L peanut oil showed a strong stimulating effect on mycelium pellet diameter and mycelial biomass;the addition of 4 g/L Tween 80 increased the yields of mycelium,and added at early stage of culture obtained a better promotion effect.

关 键 词：绣球菌 脂肪酸 表面活性剂 液体培养 

分 类 号：S646[农业科学—蔬菜学]