尿素包合法富集苦瓜籽油中α-桐酸的工艺优化  

Enrichment ofα-eleostearic acid from bitter gourd seed oil by urea adduction fractionation

作　　者：帅晓艳 王惠玲 张瑞[1,2] 杨宁 吴慕慈[1,2] 叶树芯 李玉保 何静仁 SHUAI Xiaoyan;WANG Huiling;ZHANG Rui;YANG Ning;WU Muci;YE Shuxin;LI Yubao;HE Jingren(Modern Industry School of Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Yunhong Group Corporation,Wuxue 435406,Hubei,China)

机构地区：[1]武汉轻工大学硒科学与工程现代产业学院,武汉430023 [2]武汉轻工大学食品科学与工程学院,武汉430023 [3]运鸿集团股份有限公司,河北武穴435406

出　　处：《中国油脂》2022年第4期72-76,共5页China Oils and Fats

基　　金：湖北省果蔬精深加工及营养功能食品开发企校联合创新中心(whpu-2020-kj-304)。

摘　　要：以超临界CO;萃取的苦瓜籽油为原料,经皂化酸解法制得混合脂肪酸,再采用尿素包合法富集其中的α-桐酸。在单因素试验的基础上,采用正交试验对富集工艺条件进行优化。结果表明,α-桐酸的最佳富集工艺条件为:采用95%乙醇作为尿素溶剂,混合脂肪酸与尿素质量比1∶3,尿素与95%乙醇质量比1∶5,包合温度4℃,包合时间24 h。在最佳工艺条件下,α-桐酸纯度从苦瓜籽油中的28.83%提高至60.03%,产物中共轭亚麻酸相对含量达到71.28%。Using bitter gourd seed oil extracted by supercritical CO;as raw material,the mixed fatty acid was obtained by saponification and acid hydrolysis,and then theα-eleostearic acid was enriched by urea adduction fractionation.Based on single factor experiment,the enrichment conditions were optimized by orthogonal experiment.The results showed that the optimal process for enrichingα-eleostearic acid from bitter gourd seed oil were obtained as follows:with 95%ethanol as urea solvent,mass ratio of mixed fatty acid to urea 1∶3,mass ratio of urea to 95%ethanol 1∶5,adduction temperature 4℃and adduction time 24 h.Under these conditions,the purity ofα-eleostearic acid increased from 28.83%(in bitter gourd seed oil)to 60.03%,the total relative content of conjugated linolenic acid in the product reached 71.28%.

关 键 词：苦瓜籽油 共轭亚麻酸 α-桐酸 尿素包合法 富集纯化 

分 类 号：TS225.6[轻工技术与工程—粮食、油脂及植物蛋白工程] O658[轻工技术与工程—食品科学与工程]