水稻直链淀粉质量分数快速检测方法研究--以云南元江水稻为例  

Study on Rapid Detection Method of Amylose Content in Rice:A Case Study ofYuanjiang Rice in Yunnan Province

作　　者：郑洪健 刘凯丽 徐晓东 刘鑫 杨娟[2] 阚望 程霞 白成星 殷富有[2] ZHENG Hongjian;LIU Kaili;XU Xiaodong;LIU Xin;YANG Juan;KAN Wang;CHENG Xia;BAI Chengxing;YIN Fuyou(School of Agriculture and Life Sciences,Kunming University,Kunming,Yunnan,China 650214;Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan,China 650205;Rice Research Group,Yuanjiang County Rural Industry Development Center,Yuanjiang,Yunnan,China 653300)

机构地区：[1]昆明学院农学与生命科学学院,云南昆明650214 [2]云南省农业科学院生物技术与种质资源研究所,云南昆明650205 [3]元江县乡村产业发展中心水稻研究组,云南元江653300

出　　处：《昆明学院学报》2022年第3期83-86,共4页Journal of Kunming University

基　　金：院士专家工作站(202005AF150032);云南省种子种业联合实验室、云南省青年拔尖人才专项资助项目(YNWR-QNBJ-2018-284);云南省基础研究专项面上资助项目(202001AT070015).

摘　　要：为实现对水稻中直链淀粉质量分数的快速检测,开展检测方法的研究.以元江早、晚稻为测试对象,通过优化直链淀粉质量分数测量装置选择,结合基于定量分析的直链淀粉光谱数据预处理,近红外分析的淀粉质量分数差异检测和检测结果快速校正及优化,构建了一种快速检测直链淀粉质量分数的方法.与传统“碘比色法”比对结果显示,该检测方法测定结果与“碘比色法”基本一致;检测速率达到2份/min以上,而“碘比色法”仅为1份/min.说明该方法对元江早、晚稻直链淀粉质量分数的检测能够满足精度和效率的要求.In order to realize a rapid detection of amylose content in rice with high precision and efficiency,a detection method was studied.Taking Yuanjiang early and late rice as the test object,by improving the selection of amylose mass fraction measurement device,preprocessing amylose spectral data based on quantitative analysis,detecting the difference of starch mass fraction by near-infrared analysis besides quickly correcting and optimizing the detection results,a detection method for rapid detection of amylose mass fraction was constructed.The comparison results with the traditional method“iodine colorimetry”showed that the determination results of this method were basically consistent with that of“iodine colorimetry”.The detection rate was more than 2 phr/min.In contrast,the detection rate of“iodine colorimetry”was only 1 phr/min.It showed that this detection method for the early and late rice in Yuanjiang can meet the requirements of accuracy and efficiency.

关 键 词：水稻 直链淀粉 检测方法 碘比色法 

分 类 号：TS210.4[轻工技术与工程—粮食、油脂及植物蛋白工程]