水杨酸/季鏻盐双改性壳聚糖抗菌保鲜剂的制备及表征  

Preparation and Characterization of Salicylic Acid/Quaternary Phosphonium Salt-modified Chitosan as an Antibacterial Preservative

作　　者：阮湘梅 杨子明 李普旺 张怡[1,3] 周闯[2] 何祖宇 刘运浩 宋书会 王超 RUAN Xiangmei;YANG Ziming;LI Puwang;ZHANG Yi;ZHOU Chuang;HE Zuyu;LIU Yunhao;SONG Shuhui;WANG Chao(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350000,China;Institute of South Subtropical Crops,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091,China;Engineering Research Center of Fujian and Taiwan Specialty Marine Food Processing and Nutrition Health,Ministry of Education,Fuzhou 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350000 [2]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [3]闽台特色海洋食品加工及营养健康教育部工程研究中心,福建福州350002

出　　处：《现代食品科技》2022年第6期145-151,115,共8页Modern Food Science and Technology

基　　金：海南省科技厅科技项目(ZDYF2021XDNY145);云南省李普旺专家工作站(202005AF150007);中国热带农业科学院基本科研业务费专项资金(1630062022006,1630062022023);广东省现代农业产业技术体系创新团队建设专项资金(2019KJ140);广东省农业科技创新体系管理项目(0835-190Z22404211C1)。

摘　　要：该研究以天然高分子壳聚糖(CS)为基体,首先分别用水杨酸(SA)和季鏻盐(TPPB)接枝改性壳聚糖,通过单因素实验确定壳聚糖和水杨酸、季鏻盐的最佳摩尔比,得到SA-CS和TPPB-CS聚合物,再以水杨酸壳聚糖衍生物为底物,加入不同摩尔比的季鏻盐,通过水溶性分析选出最佳摩尔比,得到SA-CS-TPPB聚合物;并通过红外光谱、核磁共振、X射线衍射和热重分析等对所得到的三个聚合物进行结构性能表征,最后通过抑菌圈实验分析SA-CS、TPPB-SA和SA-CS-TPPB聚合物的抗菌性能。结果表明,最优反应摩尔比CS:TPPB:SA为1:1:0.75;所得壳聚糖衍生物有很好的溶解性和抗菌性,溶解性可达100.00%,对金黄色葡萄球菌和大肠杆菌有很好的抑制效果,且对金黄色葡萄球菌的抑制效果优于大肠杆菌。SA对提高CS抗菌性要比TPPB效果好,季鏻盐对提高壳聚糖水溶性比水杨酸好,采用水杨酸和季鏻盐共同改性壳聚糖对提高其水溶性和抗菌性有协同增效作用,所得壳聚糖衍生物比壳聚糖有更好的成膜性能,为壳聚糖改性在果蔬涂膜保鲜中的应用提供理论依据。Natural polymer chitosan(CS)was modified using salicylic acid(SA),then quaternary phosphonium salt(TPPB).Single-factor experiments were then carried out to determine the optimal molar ratios of CS to SA and TPPB and to acquire SA-CS and TPPB-CS polymers.Following this,SA-CS derivatives were adopted as substrates and mixed with TPPB at different molar ratios to obtain the optimal molar ratio based on the water solubility results.Eventually,SA-CS-TPPB polymers were obtained.The three different polymers were subjected to infrared spectroscopy,nuclear magnetic resonance,X-ray diffraction,and thermogravimetric analysis to analyze their structures and properties.Finally,according to the zone of inhibition results,the antibacterial performances of SA-CS,TPPB-CS,and SA-CS-TPPB were examined.The results demonstrate that the optimal molar ratio(CS:TPPB:SA)is 1:1:0.75 and all the CS derivatives exhibit excellent solubility and antibacterial performances.Their solubility can reach up to 100.00%,and they show satisfactory inhibitory effects against Escherichia coli but even more so against Staphylococcus aureus.SA can better enhance the antibacterial performance of CS compared to TPPB,whereas TPPB is more effective at increasing CS solubility than SA.By modifying CS using both SA and TPPB,the solubility and antibacterial activity of CS can be improved synergistically.The CS derivatives acquired also have better film forming characteristics than CS alone.These findings are of theoretical importance for future CS modification and its application as a coating for fruit and vegetable preservation.

关 键 词：改性壳聚糖 保鲜 抗菌 季鏻盐 水杨酸 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]