铅胶体金免疫层析试纸条研制及其在小龙虾中的应用  

Development of lead ions immuno-colloidal gold chromatographic test strip and its application in Procambarus clarkii

作　　者：李杨阳 曲文莉 杨庆 段烁[1,2,3] 方敏 王桥 刘言[1,2,3] 宫智勇 许琳 LI Yang-Yang;QU Wen-Li;YANG Qing;DUAN Shuo;FANG Min;WANG Qiao;LIU Yan;GONG Zhi-Yong;XU Lin(College of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Key Laboratory for Deep Processing of Major Grain and Oil,Ministry of Education,Wuhan 430023,China;Hubei Key Laboratory for Processing and Transformation of Agricultural Products,Wuhan 430023,China)

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023 [2]大宗粮油精深加工教育部重点实验室,武汉430023 [3]湖北省农产品加工与转化重点实验室,武汉430023

出　　处：《食品安全质量检测学报》2022年第8期2413-2418,共6页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2019YFC1606000);武汉轻工大学校立科研项目(2022Y10)。

摘　　要：目的 利用胶体金免疫层析技术研制重金属铅检测试纸条,并探究其在小龙虾样品中的应用。方法 使用胶体金和铅单克隆抗体制备免疫探针。优化反应体系,构建重金属铅免疫层析检测试纸条。验证该试纸条的检出限、特异性、准确性和稳定性,并对小龙虾样品进行检测。结果 所研制的重金属铅胶体金免疫层析试纸条的检出限为6 ng/mL,检测时间为15 min。该试纸条可特异性识别铅,与其他常见重金属无交叉反应。结论 本研究研制的试纸条性能良好,能够准确判定小龙虾中重金属铅是否超标。Objective To establish a test strip for heavy metal lead ions based on colloidal gold immunochromatography and explore its application in Procambarus clarkii samples. Methods Colloidal gold and anti-lead monoclonal antibody were used to prepare the immune probes. Reaction system was optimized to develop immuno-colloidal gold chromatographic test strip for heavy metal lead ions. The limit of detection, specificity, accuracy and stability of the test strip were verified, and the Procambarus clarkii samples were detected. Results The limit of detection of the developed immuno-colloidal gold chromatographic test strip for heavy metal lead ions was 6 ng/mL,and the detection time was 15 min. The lead ions could be specifically recognized, but there was no cross-reaction to other common heavy metals. Conclusions The test strip developed in this study provides good performance and can accurately determine excessive content of heavy metal lead ions in Procambarus clarkii.

关 键 词：铅 小龙虾 胶体金免疫层析试纸条 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]