Difference analysis of different parts of chicory based on HPLC fingerprint and multi-component content determination  

作　　者：Mengzhen Yan Zhenling Zhang Yanze Liu 

机构地区：[1]School of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China [2]Henan Research Center for Special Processing Technology of Chinese Medicine,Zhengzhou 450046,China [3]Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Ministry of Education,Henan University of Chinese Medicine,Zhengzhou 450046,China [4]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China

出　　处：《Chinese Herbal Medicines》2022年第2期317-323,共7页中草药（英文版）

基　　金：financial support of the industry special project for the public welfare of the State Administration of Traditional Chinese Medicine of China(00104296)。

摘　　要：Objective:To establish HPLC fingerprints of different parts of chicory stems,leaves,roots,flowers and seeds,and compare the similarities and differences of chemical components in different parts,so as to provide a scientific basis for the comprehensive utilization of chicory.Methods:To establish the HPLC fingerprint of chicory,the chromatographic column was chosen with Agilent ZORBAX Eclipse XDB-C_(18),the mobile phase was methanol(A)-0.2%formic acid(B),the flow rate was 1 mL/min,the column temperature was 30℃,and the detection wavelength was 254 nm.The Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)was used to evaluate the similarity of different parts of decoction pieces,and the determination method of multi-component content was established based on fingerprint identification chromatographic peaks,and the determination results were analyzed.Results:The HPLC fingerprinting method of chicory was established.Sixteen chromatographic peaks were identified and 10 of them were identified as:caftaric acid(1),esculin(2),chlorogenic acid(3),esculetin(4),caffeic acid(5),cichoric acid(8),hyperoside(11),rutin(12),isochlorogenic acid C(14)and luteolin(16).The similarity of different parts was 0.084–0.701.At the same time,the total content of detected chemical components was ranked as flower>leaf>stem>root>seed.Roots did not contain caftaric acid,rutin,and luteolin,flowers did not contain luteolin,and seeds did not contain caftaric acid,cichoric acid,and luteolin.The content of cichoric acid in leaves was the most,and esculin in flowers was the most.Conclusion:The results of HPLC fingerprint and multi-component content determination revealed the similarity and difference of different parts of chicory from chemical composition,indicating that there were certain differences in different parts of chicory.The established HPLC fingerprinting method can provide a reference for quality control and evaluation of different parts of the chicory.

关 键 词：caffeic acid cichoric acid CHICORY chlorogenic acid ESCULETIN fingerprint HPLC HYPEROSIDE isochlorogenic acid C LUTEOLIN multi-component content determination RUTIN 

分 类 号：R284.1[医药卫生—中药学]