Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen  

作　　者：Jia Luo Pei-Quan Zhao Hao-Jie Chen Miao-Miao Liu Jia-Qi He Ping Fei 

机构地区：[1]Department of Ophthalmology,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China [2]Department of Urology,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China [3]department of General Surgery,Huadong Hospital,Fudan University,Shanghai 200040,China

出　　处：《International Journal of Ophthalmology(English edition)》2022年第6期868-875,共8页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81770963;No.81770964)。

摘　　要：AIM:To investigate the role of procollagen C-proteinase enhancer 1(PCPE1)in retinal angiogenesis and relevant mechanisms.METHODS:The Pcolce1-knockout(KO)mice were used to explore the effect of PCPE1 on retinal angiogenesis in vivo.Pcolce1 si RNA were designed,cell count kit 8(CCK8)assays and tube formation assays were performed to investigate the cell proliferation and tube formation abilities of retinal microvascular endothelial cells(h RMECs)in vitro.Mouse embryo fibroblasts(MEF)cells were isolated and cultured to analyze the effect of PCPE1 on enhancing procollagen cleavage.RESULTS:In vivo studies showed that the retinal vascular density of Pcolce1-/-mice was significantly lower than that of the control group.Furthermore,silencing of Pcolce1 inhibited cell proliferation and tube formation abilities of h RMECs in vitro.Additionally,much more procollagen was found in Pcolce1-/-MEF cells,compared to wild type MEF cells.CONCLUSION:PCPE1 may promote physiological retinal angiogenesis by regulating the processing of collagen,which may provide a potential therapeutic target of retinal vascular disease.

关 键 词：retinal diseases ANGIOGENESIS procollagen C-proteinase enhancer 1 COLLAGEN 

分 类 号：R77[医药卫生—眼科]