Soxllb regulates the migration and fate determination of Müller glia-derived progenitors during retina regeneration in zebrafish  

作　　者：Kaida Song Zihao Lin Lining Cao Bowen Lu Yuxi Chen Shuqiang Zhang Jianfeng Lu Hui Xu 

机构地区：[1]Key Lab of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Nantong University,Nantong,Jiangsu Province,China [2]Shanghai YangZhi Rehabilitation Hospital(Shanghai Sunshine Rehabilitation Center),Frontier Science Center for Stem Cell Research,School of Life Sciences and Technology,Tongji University,Shanghai,China

出　　处：《Neural Regeneration Research》2023年第2期445-450,共6页中国神经再生研究（英文版）

基　　金：supported by the National Key Research and Development Project of China,Nos.2017YFA0104100(to JL),2017YFA0701304(to HX);National Natural Science Foundation of China Nos.81970820(to HX),31930068(to JL)。

摘　　要：The transcription factor Sox11 plays important roles in retinal neurogenesis during vertebrate eye development.However,its function in retina regeneration remains elusive.Here we report that Sox11 b,a zebrafish Sox11 homolog,regulates the migration and fate determination of Müller glia-derived progenitors(MGPCs)in an adult zebrafish model of mechanical retinal injury.Following a stab injury,the expression of Sox11 b was induced in proliferating MGPCs in the retina.Sox11 b knockdown did not affect MGPC formation at 4 days post-injury,although the nuclear morphology and subsequent radial migration of MGPCs were alte red.At 7 days post-injury,Sox11 b knockdown res ulted in an increased proportion of MGPCs in the inner retina and a decreased propo rtion of MGPCs in the outer nuclear layer,compared with controls.Furthermore,Sox11 b knockdown led to reduced photoreceptor regeneration,while it increased the numbe rs of newborn amacrines and retinal ganglion cells.Finally,quantitative polymerase chain reaction analysis revealed that Sox11 b regulated the expression of Notch signaling components in the retina,and Notch inhibition partially recapitulated the Sox11 b knockdown phenotype,indicating that Notch signaling functions downstream of Sox11 b.Our findings imply that Sox11 b plays key roles in MGPC migration and fate determination during retina regeneration in zebrafish,which may have critical im plications for future explorations of retinal repair in mammals.

关 键 词：cell migration fate determination Müllerglia Müller glia-derived progenitor Notch signaling photoreceptor retina regeneration Sox11 transcription factor ZEBRAFISH 

分 类 号：R774[医药卫生—眼科]