多黏菌素B发酵培养基研究  

Study on the fermentation medium of polymyxins B

作　　者：姜明星 随子华 周鑫 JIANG Mingxing;SUI Zihua;ZHOU Xin(Huasheng Company of NCPC,Shijiazhuang 052160,China)

机构地区：[1]华北制药华胜公司,河北石家庄052160

出　　处：《发酵科技通讯》2022年第2期69-72,共4页Bulletin of Fermentation Science and Technology

摘　　要：多黏菌素B属多肽类抗生素,由多个结构相似的环脂肽组成,包含多黏菌素B_(1)、B_(2)、B_(3)、B_(1-I)及其他未知组分,其中B_(1)和B_(2)为有效成分,B_(3)和B_(1-I)为主要杂质。B_(1)、B_(2)、B_(3)及B_(1-I)的骨架结构相同,仅在取代基上有所不同。采用均匀设计法对多黏菌素B发酵培养基进行优化,研究培养基中添加氨基酸对组分和杂质的影响。使用DPS设计软件对实验数据进行分析,得到不同种类氨基酸对各组分和杂质的相关性。Polymyxins B is a polypeptide antibiotic,which is composed of several cyclolipids with similar structure,mainly including polymyxins B_(1),B_(2),B_(3),B_(1-I) and other unknown components.B_(1) and B_(2) are the active components,B_(3) and B_(1-I) are the main impurities.As the skeleton structures of B_(1),B_(2),B_(3) and B_(1-I) are the same,and only the substituents are different.We used the uniform design method to optimize the fermentation medium of polymyxins B,and studied the effect of adding amino acids to the medium on the components and impurities.DPS experimental design software was used to analyze the experimental data and obtain the correlation of different types of amino acids on each component and impurity.

关 键 词：多黏菌素B 培养基 氨基酸 均匀设计法 

分 类 号：Q815[生物学—生物工程]