TGF-β、PD-L1及PI3K蛋白在胃癌组织中的表达及临床意义  

Expression and clinical significance of TGF-β,PD-L1 and PI3K in gastric cancer tissues

作　　者：周康杰 胡楠 吴学雨 赖唤 洪义东 张竞舟 吴风雷[1] ZHOU Kangjie;HU Nan;WU Xueyu;LAI Huan;HONG Yidong;ZHANG Jingzhou;WU Fenglei(Lianyungang Clinical College of Nanjing Medical University,Jiangsu Lianyungang 222000,China)

机构地区：[1]南京医科大学连云港临床医学院,江苏连云港222000

出　　处：《现代肿瘤医学》2022年第13期2394-2399,共6页Journal of Modern Oncology

基　　金：国家自然科学基金青年科学基金项目(编号:81802347);江苏省社会发展面上项目(编号:BE2019693);吴阶平医学基金项目(编号:320.6750.19058);江苏省研究生科研与实践创新计划项目(编号:SJCX21_0655)。

摘　　要：目的:检测胃癌组织中TGF-β、PD-L1及PI3K的表达情况,并探讨TGF-β、PD-L1及PI3K表达的临床意义。方法:收集2017年01月至2018年01月我院74例接受过胃癌根治术且组织学证实为胃腺癌患者的病例蜡块标本。采用免疫组织化学法检测其中TGF-β、PD-L1以及PI3K的表达情况,分析其与临床病理参数之间的关系及三者在胃癌组织中表达的相关性,并分析其与胃癌患者预后生存之间的关系。结果:胃癌组织中TGF-β、PD-L1、PI3K阳性率高于癌旁组织,差异具有统计学意义(P<0.05);胃癌组织中TGF-β的表达与淋巴结转移、临床TNM分期有关(P<0.05),与年龄、性别、分化程度、浸润程度、肿瘤直径无显著相关性(P>0.05);胃癌组织中PD-L1和PI3K的表达与肿瘤浸润程度、淋巴结转移、临床TNM分期有关(P<0.05),与年龄、性别、分化程度、肿瘤直径无显著相关性(P>0.05);在胃癌组织中,TGF-β与PD-L1的表达呈正相关(P<0.05),TGF-β与PI3K的表达呈正相关(P<0.05),PI3K与PD-L1的表达呈正相关(P<0.05)。Kaplan-Meier生存分析提示,与TGF-β、PD-L1、PI3K阴性相比,TGF-β、PD-L1、PI3K阳性表达的患者总生存期明显缩短(P<0.05)。结论:胃癌组织中存在TGF-β、PD-L1以及PI3K的高表达,并且TGF-β、PD-L1、PI3K的表达与淋巴结转移、临床TNM分期密切相关,三者可能参与胃癌的发生、发展和转移,且高表达患者的预后情况较差。Objective:To investigate the expressions of TGF-β,PD-L1 and PI3K in gastric cancer tissues,and explore the clinical significance of TGF-β,PD-L1 and PI3K expressions.Methods:The paraffin tissue samples and clinicopathological features of 74 patients who underwent gastrectomy from January 2017 to January 2018 were collected.The expression of TGF-β,PD-L1 and PI3K in gastric cancer and adjacent tissues were investigated by immunohistochemistry,and the relationship between them and the clinicopathological parameters of gastric cancer was analyzed,as well as the correlation between the three expression in gastric cancer tissues were analyzed.The correlation between TGF-β,PD-L1 and PI3K and the prognosis of gastric cancer patients were also analyzed.Results:The positive rates of TGF-β,PD-L1 and PI3K in gastric cancer tissues were higher than those in adjacent tissues,and the difference was statistically significant(P<0.05).The expression of TGF-βin gastric cancer was associated with lymph node metastasis and clinical TNM stage(P<0.05)and there was no significant correlation with age,gender,degree of differentiation,degree of invasion and tumor diameter(P>0.05).The expression of PD-L1 and PI3K in gastric cancer was correlated with the degree of tumor invasion,lymph node metastasis and clinical TNM stage(P<0.05),and there was no significant correlation with age,gender,degree of differentiation and tumor diameter(P>0.05).TGF-βwas positively correlated with PD-L1 expression in gastric cancer tissues(P<0.05).TGF-βwas positively correlated with PI3K expression(P<0.05).PI3K was positively correlated with PD-L1 expression(P<0.05).Kaplan-Meier survival analysis showed that compared with negative expression of TGF-β,PD-L1 and PI3K,patients with positive expression of TGF-β,PD-L1 and PI3K had significantly shorter overall survival(P<0.05).Conclusion:TGF-β,PD-L1 and PI3K showed high expression in gastric cancer tissues and the expression of TGF-β,PD-L1 and PI3K were closely related to some clinicopathological features s

关 键 词：胃癌 免疫检查点阻断治疗 TGF-Β PD-L1 PI3K 

分 类 号：R735.2[医药卫生—肿瘤]