一株高产纤维素酶真菌的分离与鉴定  

Isolation and Identification of a High Efficiency Cellulose Degrading Fungus

作　　者：尹守亮 杨镒婴 李秋园 杨何宝 郭世奇 周志江[1] YIN Shou-liang;YANG Yi-ying;LI Qiu-yuan;YANG He-bao;GUO Shi-qi;ZHOU Zhi-jiang(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;School of Life Sciences,North China University of Science and Technology,Tangshan 063210,China;Postdoctoral Innovation Practice Base,Zhongrong Technology Co.,Ltd.,Tangshan 064499,China)

机构地区：[1]天津大学化工学院,天津300072 [2]华北理工大学生命科学学院,河北唐山063210 [3]中溶科技股份有限公司博士后创新实践基地,河北唐山064499

出　　处：《纤维素科学与技术》2022年第2期9-18,共10页Journal of Cellulose Science and Technology

基　　金：河北省自然科学基金面上项目(C2019209399);唐山市科技局项目(20130208b);华北理工大学引进人才科研启动资金(BS201833)。

摘　　要：纤维素类生物质是地球上分布最为广泛的可再生资源,筛选高效的纤维素降解菌株对于纤维素生物质资源的开发和利用具有重要的意义。采用微晶纤维素作为唯一碳源的筛选方法,从曹妃甸湿地土壤样品中分离到一株高产纤维素酶的真菌,通过对菌株形态学特征观察和18SrDNA序列比对分析,鉴定其为土曲霉种属。发酵检测该菌株粗酶液中含有较高活性的外切葡聚糖酶和β-葡萄糖苷酶,活力大小分别为110.7、167.7U/mL;其内切葡聚糖酶和滤纸酶酶活力分别为27.8、51.5U/mL,土曲霉CB10可作为潜在的开发菌种用于纤维素生物降解的研究。Cellulosic biomass is the most abundant biorenewable material on the earth.Screening efficient degrading enzymes is of great significance for the development and utilization of cellulosic biomass.A fungal species with high cellulase was isolated from soil samples in Caofeidian using the method of avicel as the only carbon source.The strain was identified as Aspergillus terreus based on the morphological features and 18S rDNA sequence comparison analysis.The exoglucanase andβ-glucosidase activities of the fermentation supernatant were 110.7 U/m L and 167.7 U/mL,respectively,and the endo-β-glucanase and filter paper activities were 27.8 U/mL and 51.5 U/mL,respectively.The strain CB10 could be used as a potential resource for the biodegradation of cellulose.

关 键 词：纤维素 唯一碳源 纤维素酶 形态特征 18S rDNA 土曲霉 酶学活性 

分 类 号：Q939.9[生物学—微生物学]