光谱对小黄鱼胚胎在孵化、关键免疫因子水平及感光和节律基因表达的影响分析  

The Spectral Influence Analysis of Embryo Incubation,Vital Immune Factors Level,Photosensitive and Rhythmical Related Gene Expression of Larimichthys polyactis

作　　者：李沪 韩明明 詹炜 刘峰 谢庆平 徐万土 楼宝 LI Hu;HAN Ming-ming;ZHAN Wei(School of Fisheries of Zhejiang Ocean University,Zhoushan 316022;Institute of Hydrobiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江海洋大学水产学院,浙江舟山316022 [2]浙江省农业科学院水生生物研究所,浙江杭州310021 [3]象山港湾苗种有限公司,浙江宁波315799

出　　处：《浙江海洋大学学报（自然科学版）》2022年第3期211-220,共10页Journal of Zhejiang Ocean University：Natural Science

基　　金：浙江省农业科学院学科项目建设经费(10419000021CC2201G);国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0901204);浙江省重点研发计划(2021C02055)。

摘　　要：通过比较6种光谱环境(自然光、红光、绿光、蓝光、白光、黑暗)下小黄鱼胚胎的孵化率、孵化时长和主要免疫因子的含量及初孵仔鱼中melanopsin和clock基因的表达,发现:(1)红光组胚胎孵化率最高(49.56%),其次为黑暗组(38.66%)、全光组(31.46%)、自然光组(31.25%)、绿光组(30.72%)和蓝光组(29.58%);(2)红光组和黑暗组孵化最快,全光组和自然光组次之,蓝光和绿光组最慢;(3)红光组中IgM水平最高,而C3、TGF-β和IL^(-1)β在自然光组、全光、蓝光和绿光组中的含量水平高于红光组和黑暗组;(4)蓝光组中感光蛋白melanopsin水平最高,clock表达水平最低,而红光组与之相反。结果说明光谱对小黄鱼胚胎在孵化、免疫水平和节律调控等方面均产生了显著影响。By comparing the hatching rate and incubation period of the embryo,the concentration of major immune factors and the relative expression level of melanopsin and clock in newly hatched larvae of Larimichthys polyactis treated under 6 different kinds of spectra(natural,red,green,blue,white and dark),we find:(1)The red group obtained the highest hatching rate(49.56%),followed by dark group(38.66%),full group(31.46%),natural group(31.25%),green group(30.72%)and blue group(29.58%);(2)The red and dark group incubated faster than the other groups;(3)The concentration of IgM was highest in red group,while C3,TGF-βand IL^(-1)βwere higher in natural,full,blue and green group than in red and dark group;(4)Contrary to red group,Melanopsin expressed highest in blue group,while clock existed higher in red group.All these results indicated the spectra could significantly influence the L.polyactis embryo in the aspects of incubation,immunol level and rhythm regulation.

关 键 词：光谱 小黄鱼 孵化率 孵化时长 免疫因子 MELANOPSIN CLOCK 

分 类 号：S965[农业科学—水产养殖] S917.4[农业科学—水产科学]