HPLC法测定保健食品B族维生素片中9种维生素B的含量  

Determination of Nine Kinds of Vitamin B Components in Healthy Food Vitamin B Tablets by HPLC

作　　者：谭婉清 王术玲[1] 关日晴[2] TAN Wanqing;WANG Shuling;GUAN Riqing(College of Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Guangdong Drug Inspection Institute,Guangzhou 510663,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广东省药品检验所,广东广州510663

出　　处：《食品工业科技》2022年第13期303-310,共8页Science and Technology of Food Industry

摘　　要：目的:建立高效液相色谱-二极管阵列检测(HPLC-DAD)法测定B族维生素片中9种维生素B成分的含量。方法:样品中的叶酸经0.3%氨溶液超声提取,样品中的生物素、维生素B_(12)和泛酸经水超声提取,样品中的维生素B_(1)、维生素B_(2)、维生素B_(6)、烟酸和烟酰胺经0.1%盐酸超声提取,经C_(18)柱分离,以甲醇-0.1 mol/L磷酸二氢钾溶液为流动相进行梯度洗脱,泛酸和生物素在205 nm波长处进行检测,维生素B_(1)、维生素B_(2)、烟酸和烟酰胺在256 nm波长处进行检测,维生素B_(6)和叶酸在287 nm波长处进行检测,维生素B_(12)在361 nm波长处进行检测。结果:9种成分能实现较好地分离,在各自线性范围内线性关系良好,决定系数R2均大于0.999,平均回收率为91.8%~106.9%,RSD为0.2%~4.8%,方法的检出限在0.05~4.7 mg/kg之间。结论:该方法操作快速简便、精密度和重复性好,能快速完成保健食品B族维生素片中9种维生素B成分的测定,并降低成本。Objective:To establish a method for the determination of nine vitamin B components in vitamin B tablets by high performance liquid chromatography-diode array detector(HPLC-DAD).Method:Folic acid was ultrasonically extracted with 0.3%ammonia solution,biotin,vitamin B_(12) and pantothenate acid were ultrasonically extracted with water,the vitamins B_(1),B_(2),B_(6),niacin and nicotinamide were ultrasonically extracted by 0.1%hydrochloric acid,separated by C_(18) column with methanol-0.1 mol/L potassium dihydrogen phosphate solution as the mobile phase for gradient elution.Pantothenate acid and biotin were detected at the wavelength of 205 nm,vitamins B_(1),B_(2),niacin and nicotinamide were detected at the wavelength of 256 nm,vitamins B_(6) and folic acid were detected at the wavelength of 287 nm,vitamins B_(12) was detected at the wavelength of 361 nm.Results:The results showed that the nine components could be separated well,and the linear relationship was good in their linear range.The average recoveries were 91.8%~106.9%,RSD were 0.2%~4.8%,and the detection limit of the method were 0.05~4.7 mg/kg.Conclusion:This method is rapid,simple,accurate and repeatable.It can quickly complete the determination of nine vitamin B components in health food vitamin B tablets and reduce the cost.

关 键 词：HPLC-DAD B族维生素片 维生素B_(1) 维生素B_(2) 维生素B_(6) 维生素B_(12) 烟酸 烟酰胺 叶酸 泛酸 生物素 

分 类 号：TS207.3[轻工技术与工程—食品科学]