FAD核酸适配体生物传感器的制备及其葡萄糖检测的应用  

Preparation of Flavin Adenine Dinucleotide Aptamer-Based Sensor and Its Application for Glucose Detection

作　　者：王艳[1] 王悦[1] 张帅[1] 赵宁[1] 辛嘉英[1] 孙立瑞 关桦楠[1] WANG Yan;WANG Yue;ZHANG Shuai;ZHAO Ning;XIN Jiaying;SUN Lirui;GUAN Huanan(Key Laboratory of Food Science and Engineering,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学食品科学与工程重点实验室,黑龙江哈尔滨150076

出　　处：《食品科学》2022年第12期179-186,共8页Food Science

基　　金：中央支持地方高校改革发展资金人才培养支持计划项目(高水平人才)(304017);黑龙江省自然科学基金联合引导项目(LH2020C063);哈尔滨商业大学青年创新人才项目青年后备人才专项(2019CX33)。

摘　　要：以纳米金(gold nanoparticles,AuNPs)为主体,甲烷氧化菌素(methanobactin,Mb)和黄素腺嘌呤二核苷酸(flavin adenine dinucleotide,FAD)为配体构建FAD核酸适配体传感器。利用AuNPs颗粒较强的吸附能力,将Mb包裹于纳米颗粒上,形成稳定的Mb-AuNPs结构体。采用滴涂法将Mb-AuNPs结构体修饰于裸金电极表面,借助Mb结构中的—COOH与FAD牢固结合,将FAD引入Mb-AuNPs结构体上,提高对葡萄糖的识别能力,从而构建具备葡萄糖氧化酶活性的新型核酸适配体生物传感器。通过循环伏安法及时间-电流曲线法探讨其应用于葡萄糖检测的可行性。在温度25℃、pH 7.2时,葡萄糖浓度为10~200μmol/L,与该生物传感器的响应电流存在良好的线性关系,R^(2)为0.99791,检出限为4.22×10^(-8) mol/L(R_(SN)=3)。该核酸适配体传感器具有制备简单、价格低廉、易于操作、可快速检测等优点。An flavin adenine dinucleotide(FAD)aptamer-based sensor was constructed with gold nanoparticles(AuNPs)as the main body,and methanobactin(Mb)and FAD as the ligands.Mb was used to wrap AuNPs,which has a strong adsorption capacity for Mb,to form a stable Mb-AuNPs structure.The Mb-AuNPs structure was used to modify the surface of the bare gold electrode by the drip-coating method,and FAD was introduced into the Mb-AuNPs structure,in which the–COOH groups could firmly bind to FAD,to improve the ability to recognize glucose.As a result,a novel aptamer biosensor with glucose oxidase-like activity could be constructed.Cyclic voltammetry and the time-current curve method were used to explore the feasibility of its application in glucose detection.At 25℃and pH 7.2,there was a good linear relationship between glucose concentration in the range of 10–200 μmol/L and the response current of the biosensor,with a correlation coefficient(R^(2))of 0.99791,and the detection limit was 4.22×10^(-8) mol/L(R_(SN)=3).The aptamer-based sensor has several advantages such as simple preparation,low price,easy operation and rapid detection.

关 键 词：核酸适配体生物传感器 葡萄糖氧化酶模拟酶 甲烷氧化菌素 黄素腺嘌呤二核苷酸 葡萄糖检测 

分 类 号：TS207[轻工技术与工程—食品科学]