血红密孔菌菌丝体多糖提取条件优化及活性研究  

Optimization in Extraction Technology of Polysaccharides from Pycnoporus sanguineus Mycelium and its Activity

作　　者：鲁铁 李登 朱园园 苏正娴 段鹏菲 陈怀中 刘宇 谢朝晖 LU Tie;LI Deng;ZHU Yuan-yuan;SU Zheng-xian;DUAN Peng-fei;CHEN Huai-zhong;LIU Yu;XIE Zhao-hui(College of Life Science and Bioengineering,Henan University of Urban Construction,Pingdingshan 467036,China;College of Life Science and Technology,Jinan University,Guangzhou 510632,China;Agriculture College of Ludong University,Yantai 264025,China)

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036 [2]暨南大学生命科学技术学院,广东广州510632 [3]鲁东大学农学院,山东烟台264025

出　　处：《中国食用菌》2022年第6期52-58,共7页Edible Fungi of China

基　　金：山东省重点研发计划资助项目(NO.2019GSF108165)。

摘　　要：在试验中探究了超声辅助时间、热水浸提时间、提取温度、料液比4个因素在血红密孔菌菌丝体多糖提取中的最优条件,并评估其抗氧化活性和抑菌能力。在单因素试验的基础上采用响应面法对其多糖提取工艺进行多因素试验,结果显示其提取的最优条件为超声辅助时间15 min、热水浸提时间59 min、提取温度79.7℃、料液比1∶30,此时血红密孔菌菌丝体多糖提取率为5.231%。且试验结果还表明血红密孔菌菌丝体多糖具有抗氧化活性和抑菌能力。该研究结果为进一步开发食用菌功能食品提供了基础性数据。The optimal extraction conditions of polysaccharide from the mycelium of Pycnoporus sanguineus by four factors,including extraction temperature,material-liquid ratio,extraction time and ultrasonic time were studied.The polysaccharide of antioxidant activity and antibacterial ability was detected to evaluate.On the basis of single-factor,response surface methodology was applied to extraction technology of polysaccharide about the mycelium of P.sanguineus.The results showed that the optimal extraction conditions were as follows:the ultrasonic assisted time was 15 min,the hot water extraction time was 59 min,the temperature was 79.7℃,the material liquid was 1∶30.The extraction rate of polysaccharide from P.sanguineus mycelia was 5.231%under this condition.The results also showed that mycelia polysaccharides of P.sanguineus has antioxidant activity and antibacterial ability.The study results will provide basic data for further development of edible fungi functional food.

关 键 词：血红密孔菌 工艺优化 抗氧化性 抑菌活性 

分 类 号：S646.2[农业科学—蔬菜学]