2株新型冠状病毒全基因测序及插入和终止突变分析  

Analysis on whole genome sequencing and insertion and stop mutation of 2 severe acute respiratory syndrome coronavirus 2 strains

作　　者：韩淑琪 HAN Shu-qi(Department of Laboratory,Jining City Center for Disease Control and Prevention,Jining,Shandong,272000 China)

机构地区：[1]济宁市疾病预防控制中心检验科,山东272000

出　　处：《预防医学论坛》2022年第5期330-334,共5页Preventive Medicine Tribune

基　　金：山东省医药卫生科技发展计划项目(202112060725);山东省济宁市重点计划研发项目[医学研究和临床医学类(2021071)]。

摘　　要：目的 分析2株新型冠状病毒基因进化变异情况及分子特征。方法 利用二代测序技术,对2份新型冠状病毒核酸检测阳性样本进行全基因组测序。利用生物信息学软件对基因序列进行分析。结果 获得长度分别为29 826 bp和29 837 bp的两条新型冠状病毒全基因序列,与Wuhan-Hu-1株基因序列同源性分别为99.96%和99.97%。两条序列的N蛋白均发生S194L变异,N蛋白S194磷酸化位点和N192潜在糖基化位点消失。其中序列1的3 647~3 648 bp之间插入了CCCAC序列,导致非结构蛋白3(NSP3)发生移码突变,即编码一种新的无跨膜结构、含有完整泛素样结构域的NSP3Δ蛋白。序列2发生了C27978T突变,导致开放读码框8(ORF8)发生终止突变,即编码1个新的仅含28个氨基酸的截短型ORF8蛋白。结论 新型冠状病毒进化变异方式复杂多样,插入突变和终止突变可能改变相关蛋白的结构和性质,应加大新型冠状病毒的病原学监测力度。Objective To analyze the evolution and variation characteristics of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods The second-generation sequencing technology was used to sequence the whole genome of novel coronavirus nucleic acid positive samples.The gene sequence was analyzed by bioinformatics software.Results A total of 2 sequences of SARS-CoV-2 were obtained.The homology of the sequences with Wuhan-Hu-1 strains were 99.96% and 99.97%.Both the N protein had S194 L mutation which caused the phosphorylation site of S194 and the potential glycosylation site of N192 disappeared.CCCAC sequence was inserted into 3647-3648 bp of sequence 1 which caused NSP3 occurred frameshift mutation.This sequence of SARS-CoV-2 encoded a new NSP3Δ protein which had no transmembrane structure but had complete ubiquitin-like domain.ORF8 of sequence 2 had C27978 T mutation that caused ORF8 occurred stop mutation,which encoded a new truncated ORF8 protein with 28 amino acids.Conclusion The evolution of SARS-CoV-2 is complicated.Insertion mutation and stop mutation could change the structure and properties of proteins.Etiological surveillance of SARS-CoV-2 should be strengthened.

关 键 词：新型冠状病毒 全基因组测序 突变 非结构蛋白3 开放读码框8 

分 类 号：Q527.5[生物学—生物化学] R373[医药卫生—病原生物学]