Soybeanβ-conglycinin and glycinin reduced growth performance and the intestinal immune defense and altered microbiome in juvenile pearl gentian groupers Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂  

作　　者：Yuanfa He Jinfang Liang Xiaohui Dong Hongyu Liu Qihui Yang Shuang Zhang Shuyan Chi Beiping Tan 

机构地区：[1]Laboratory of Aquatic Animal Nutrition and Feed,Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China [2]Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Center of Guangdong Province,Zhanjiang 524088,China [3]Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture,Zhanjiang 524088,China

出　　处：《Animal Nutrition》2022年第2期193-203,共11页动物营养（英文版）

基　　金：the National Key R&D Program of China(2019YFD0900200);the China Agriculture Research System of MOFand MARA(CARS-47);the National Natural Science Foundation of China(NSFC 31772864);the Fund of Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)(ZJW-2019-06)。

摘　　要：The utilization efficiency of soy protein is affected by its 2 anti-nutritional substancesdthe antigens bconglycinin and glycinin.This study investigated their effects on the growth performance,intestinal immune defense,andmicrobiome in juvenile pearl gentian groupers(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂).Three isonitrogenous and isolipidic diets were formulated containing fishmeal supplemented with 70 g/kgβ-conglycinin or 100 g/kg glycinin,or no supplementation(control).Each experimental diet was fed to quadruplicate groups with 30 fish in each tank for 8 weeks.Dietary inclusion of eitherβ-conglycinin or glycinin significantly reduced weight gain and specific growth rates,and cell proliferation of the distal intestine.Histological evaluation of the intestine tract revealed the inflammation signs,characterized by reducing of plica height and width as well as the number of the goblet cells,and widening of the lamina propria.The group fed theβ-conglycinin diet had reduced lysozyme activity,contents of immunoglobulin M and complements 3 and 4.Increased activities of caspase-3 and-9 were observed in the group fed the bconglycinin diet compared to the other 2 groups.In the intestinal microbiota,the relative abundances of the potentially pathogenic genera Photobacterium and Vibrio were significantly higher in the glycinin group than those in others.Therefore,the existence of soybean antigens(β-conglycinin or glycinin)could damage the structural integrity of the intestine,reduce immune defense,reshape the intestinal microbiome and,ultimately,impair growth in fish.

关 键 词：Β-CONGLYCININ GLYCININ Pearl gentian groupers Immune response Intestinal microbiota 

分 类 号：S965.334[农业科学—水产养殖]