喜马红景天叶绿体基因组特征及其系统发育分析  

Characteristics of Complete Chloroplast Genome and Phylogenetic Analysis of Rhodiola himalensis(Crassulaceae)

作　　者：张雨 苏旭 刘玉萍[1,2] 刘涛 郑长远[1,2] 苏丹丹 王亚男[1,2] 吕婷 ZHANG Yu;SU Xu;LIU Yuping;LIU Tao;ZHENG Changyuan;SU Dandan;WANG Yanan;LÜTing;无(School of Life Science,Qinghai Normal University,Xining 810008;Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibet Plateau in Qinghai Province,Qinghai Normal University,Xining 810008;Academy of Plateau Science and Sustainability,Qing-hai Normal University,Xining 810016)

机构地区：[1]青海师范大学生命科学学院,西宁810008 [2]青海师范大学青海省青藏高原药用动植物资源重点实验室,西宁810008 [3]青海师范大学高原科学与可持续发展研究院,西宁810016

出　　处：《植物研究》2022年第4期602-612,共11页Bulletin of Botanical Research

基　　金：科技基础资源调查专项项目(2017FY100202-02);祁连山国家公园青海研究中心开放课题;青海省青藏高原药用动植物资源重点实验室项目(2020-ZJ-Y40)。

摘　　要：以青藏高原特有药用植物——喜马红景天(Rhodiola himalensis)为试验材料,利用高通量测序技术对喜马红景天进行叶绿体基因组测序、组装和注释,获得完整的叶绿体基因组。结果显示:喜马红景天叶绿体基因组全长为151074 bp,GC含量为37.8%,具有1个长单拷贝区、1个短单拷贝区和1对反向重复区的典型四分体结构,其序列长度分别为82309、17017、25874 bp;叶绿体基因组共编码130个基因,其中编码蛋白的基因86个、编码tRNA的基因37个、编码rRNA的基因7个;叶绿体基因组共检测出25513个密码子,其中编码亮氨酸(Leu)的密码子占比最大;喜马红景天IRa和IRb区的rps19和ycf1基因缺失,长单拷贝区的trnH基因收缩;喜马红景天与圣地红景天(R.sacra)亲缘关系最近;短单拷贝区域的单核苷酸多态性(SNP)变异频率最高。本研究报道了喜马红景天的叶绿体基因组,并对其进行了组装、注释和序列分析,为今后开展喜马红景天的遗传多样性研究和合理开发利用提供理论依据。The complete chloroplast genome of Rhodiola himalensis was sequenced,assembled and annotated using high-throughput sequencing technology,and R.himalensis was used as material,an endemic medical species to the Qinghai-Tibet Plateau.The results showed that the complete chloroplast genome of R.himalensis was 151074 bp in length with a GC content of 37.8%.It had a typical tetrad structure with a long single copy region,a short single copy region and a pair of inverted repeat regions,and the sequence length of them was82309,17017 and 25875 bp,respectively.The complete chloroplast genome encoded 130 genes,including86 protein coding genes,37 tRNA genes and seven rRNA genes.Based on the chloroplast genome of R.himalensis,a total of 25513 codons was detected,among which the largest proportion of codons was Leu.In the IRa and IRb regions of R.himalensis,the gene of rps19 and ycf1 was deletion,and the gene of trnH in LSC region was contraction.And R.himalensis and R.sacra had the closest genetic relationship were also found.Besides,the variation frequency of single nucleotide polymorphism(SNP)was the highest within the SSC region.The complete chloroplast genome of R.himalensis was reported,assembled,annotated and analyzed,which could provide the theoretical basis for the study of genetic diversity and rational development and utilization of R.himalensis in the future.

关 键 词：叶绿体基因组 系统发育 简单重复序列 IR区边界分析 喜马红景天 

分 类 号：Q949.4[生物学—植物学]