人参、西洋参中多糖成分分析及其抗氧化活性研究  

Analysis of polysaccharide from ginseng and Panax quiquefolium L.and their antioxidant activity

作　　者：王国明[1] 徐清华 谢丽娟 李健豪 刘宝峰[2] 徐芳菲 WANG Guo-ming;XU Qing-hua;XIE Li-juan;LI Jian-hao;LIU Bao-feng;XU Fang-fei(Jilin Ginseng Research Institute,Changchun 130033,Jilin,China;Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,Jilin,China)

机构地区：[1]吉林人参研究院,吉林长春130033 [2]中国科学院长春应用化学研究所,吉林长春130022

出　　处：《粮食与油脂》2022年第6期143-146,150,共5页Cereals & Oils

基　　金：吉林省卫生健康委员会项目(2019003);吉林省行业科研专项课题(吉科发财[2017]27号)。

摘　　要：采用传统水提法、快速溶剂萃取(FSE)技术提取人参、西洋参多糖,并通过气相色谱-质谱联用(GC-MS)分析其中的单糖组成,比较2种多糖的体外抗氧化活性。结果表明:人参及西洋参粗多糖得率为FSE法>传统水提法;GC-MS分析检测出人参多糖及西洋参多糖由均由阿拉伯糖、鼠李糖、岩藻糖、木糖、甘露糖、半乳糖、葡萄糖组成,但在含量上有一定的差异;人参多糖和西洋参多糖均具有一定的DPPH·和·OH体外清除能力且呈剂量依赖性,进而为人参、西洋参多糖的生物活性研究及开发应用提供了理论基础。The polysaccharides of ginseng and Panax quiquefolium L.were extracted by traditional water extraction and fast solvent extraction(FSE),and their monosaccharide compositions were analyzed by gas chromatography-mass spectrometry(GC-MS),and their antioxidant activities in vitro were compared.The results showed that the yield of crude polysaccharides from ginseng and Panax quiquefolium L.was FSE method>traditional water extraction method;GC-MS analysis showed that both ginseng polysaccharide and Panax quiquefolium L.polysaccharide were composed of arabinose,rhamnose,fucose,xylose,mannose,galactose and glucose,but there were some differences in content.Both ginseng polysaccharide and Panax quiquefolium L.polysaccharide have certain DPPH·and·OH scavenging capacity in vitro in a dose-dependent manner,which provided a theoretical basis for the biological activity research,development and application of ginseng and Panax quiquefolium L.polysaccharides.

关 键 词：人参 西洋参 多糖 快速溶剂萃取 单糖 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]