Lunasin peptide promotes lysosome-mitochondrial mediated apoptosis and mitotic termination in MDA-MB-231 cells  

作　　者：Yuqiong Hao Huimin Guo Yechun Hong Xin Fan Yumei Su Xiushi Yang Guixing Ren 

机构地区：[1]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Institute of Plant and Food Science,Department of Biology,Southern University of Science and Technology,Shenzhen 518055,China [3]Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China [4]College of Life Sciences,Xinjiang Agricultural University,Urumqi 830001,China [5]Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,China

出　　处：《Food Science and Human Wellness》2022年第6期1598-1606,共9页食品科学与人类健康（英文）

基　　金：financially supported by the Agricultural Science and Technology Innovation Program[CAAS-ASTIP-2021-ICS]。

摘　　要：Lunasin,a novel bioactive peptide,is well-known for its anti-proliferation activity.However,the mechanism of this effect is still poorly reported.Here,synthesized lunasin was used and its anti-proliferative function was observed at the concentration of 0.25 mg/m L in human breast cancer cell MDA-MB-231.Conjoint analysis of transcriptome and proteome of MDA-MB-231 cells was further performed.The results demonstrated that cysteinyl aspartate specific proteinase(CASP)3,CASP 7,and CASP 14 were significantly up-regulated after lunasin exposure,together with an increased Bax/Bcl-2 ratio from 22.9 to 210.6,which indicated that caspase-mediated mitochondria intrinsic apoptosis was highly activated.Moreover,lysosomal pathway was signifi cantly suppressed under lunasin exposure,suggesting that lysosome may cooperate with mitochondria to participate in apoptosis.In addition,lunasin also down-regulated genes involved in DNA replication in MDA-MB-231 cells.Overall,our study reveals that the anti-proliferation effect of lunasin peptide might be triggered via the inhibition of DNA replication and cell mitosis,as well as the promotion of lysosome-mitochondrial mediated cell apoptosis.

关 键 词：ANTI-PROLIFERATION Transcriptome and proteome analysis Cell apoptosis DNA replication 

分 类 号：TS201.4[轻工技术与工程—食品科学]