Production of antihypertensive and antidiabetic peptide fractions from quinoa(Chenopodium quinoa Willd.)by electrodialysis with ultrafi ltration membranes  

作　　者：Adrián González-Muñoz Marion Valle Rotimi E.Aluko Laurent Bazinet Javier Enrione 

机构地区：[1]Biopolymer Research and Engineering Lab(BiopREL),School of Nutrition and Dietetics,Faculty of Medicine,Universidad de los Andes,MonseñorÁlvaro del Portillo 12455,Chile [2]Centre for Biomedical Research and Innovation(CIIB),Universidad de los Andes,Chile,MonseñorÁlvaro del Portillo 12455,Chile [3]Institute of Nutrition and Functional Foods(INAF),UniversitéLaval,Québec QC G1V 0A6,Canada [4]Department of Anatomy and Physiology,Faculty of Medicine,Laval Hospital Research Center,UniversitéLaval,Québec QC G1V 4G5,Canada [5]Department of Food and Human Nutritional Sciences,University of Manitoba,Winnipeg MB R3T 2N2,Canada [6]Department of Food Sciences,Laboratoire de Transformation Alimentaire et ProcédésÉlectro Membranaire(LTAPEM,Laboratory of Food Processing and Electro-Membrane Processes),UniversitéLaval,Québec QC G1V 0A6,Canada

出　　处：《Food Science and Human Wellness》2022年第6期1650-1659,共10页食品科学与人类健康（英文）

基　　金：financially supported by the Postdoctoral Fellowship N°3190683 of Dr.Adrián González-Munoz from the Chilean Agencia Nacional de Investigación y Desarrollo(ANID);the Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery Grant Program(Grant SD RGPIN-2018-04128 of Prof.Laurent Bazinet)。

摘　　要：Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes(EDUF)have gained attention since it can fractionate in terms of their charge and molecular weight.Quinoa is a pseudo-cereal highlighted by its high protein content,amino acid profile and adapting growing conditions.The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity.Experimental data showed the production of peptides ranging between 0.4 and 1.5 k Da.Cationic(CQPF)(3.01%),anionic(AQPF)(1.18%)and the electrically neutral fraction quinoa protein hydrolysate(QPH)-EDUF(~95%)were obtained.In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF(17%and 11%)indicating potential antidiabetic activity.The antihypertensive effect studied in-vivo in spontaneously hypertensive rats(SHR),showed a decrease in systolic blood pressure in presence of the fractionated peptides,being 100 mg/kg a dose comparable to Captopril(positive control).These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions.Nevertheless,further characterization is needed towards peptide sequencing,their respective role in the metabolism and scaling-up production using EDUF.

关 键 词：QUINOA Electrodialysis with ultrafiltration membranes Bioactive peptides ANTIHYPERTENSIVE ANTIDIABETIC 

分 类 号：TS210.4[轻工技术与工程—粮食、油脂及植物蛋白工程]