A Preliminary Gas Chromatography‑Mass Spectrometry‑Based Metabolomics Study of Rats Ingested Diazepam or Clonazepam  

作　　者：Shiyong Fang Jianxia Chen Xinhua Dai Yuzi Zheng Hao Wu Yingqiang Fu Jian Li Yi Ye Linchuan Liao 

机构地区：[1]Department of Forensic Analytical Toxicology,West China School of Basic Medical Sciences and Forensic Medicine,Sichuan University,Chengdu,Sichuan,China These authors contributed equally to this work

出　　处：《Journal of Forensic Science and Medicine》2020年第4期117-125,共9页法庭科学与法医学杂志（英文）

基　　金：The study was financially supported by the Project of the National Natural Sciences Foundation of China(81373239);The Innovation and Business Starting-oriented training program of College Students in Sichuan Province(C2020113713).

摘　　要：Drug-facilitated sexual assault(DFSA)is a sexual act in which the victim is unable to give or rescind consent due to alcohol or drug intoxication,which involved the abuse of benzodiazepines around the world.Conventional techniques used for the analysis of benzodiazepines have the limitation of short detection time window due to the rapid metabolism of these drugs in body.This study aimed to investigate the characteristic changes of metabolites in the blood of rats after ingesting diazepam/clonazepam through a gas chromatography-mass spectrometry-based metabolomics method,allowing the indirect reveal of the rats ingested diazepam/clonazepam.First,we found that diazepam and clonazepam in the blood of rats could not be detected by liquid chromatography-tandem mass spectrometry after 48 h of ingestion.Then,orthogonal partial least squares discrimination analysis regression models were,respectively,constructed to determine whether the rats ingested diazepam/clonazepam after 48 h.The results showed that 5 metabolites were found to be associated with diazepam exposure,and 7 metabolites were found to be associated with clonazepam exposure,which may be characterization for the evaluation of digestion of diazepam and clonazepam in rat.

关 键 词：CLONAZEPAM DIAZEPAM gas chromatography-mass spectrometry liquid chromatography-tandem mass spectrometry metabolomics 

分 类 号：R28[医药卫生—中药学]