蒸大蒜多糖抗幼年大鼠菌群失调性腹泻的作用及机制  

Effect and Mechanism of Steamed Allii Sativi Bulbus Polysaccharide Against Dysbiosis-induced Diarrhea in Young Rats

作　　者：李亚荣 郭梦雨 于垠廷 周浓 李伟东[1] LI Yarong;GUO Mengyu;YU Yinting;ZHOU Nong;LI Weidong(Jiangsu Provincial Key Laboratory of Chinese Medicine Processing,School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404020,China)

机构地区：[1]南京中医药大学药学院,江苏省中药炮制重点实验室,南京210023 [2]重庆三峡学院生物与食品工程学院,重庆404020

出　　处：《中国实验方剂学杂志》2022年第13期77-84,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81773902,81973484)。

摘　　要：目的:验证蒸大蒜多糖抗幼年大鼠菌群失调性腹泻的药效,采用16S rRNA技术探究其对幼年菌群失调性腹泻大鼠肠道菌群的调节作用。方法:将SD幼年大鼠随机分为空白组、模型组、双歧杆菌三联活菌胶囊组(西药组)、蒸大蒜多糖高剂量组、低剂量组,每组6只。建立菌群失调性腹泻模型,正常组与模型组给予生理盐水灌胃,给药剂量均为10 mL·kg^(-1),高剂量组给予500 mg·kg^(-1)蒸大蒜多糖,低剂量组给予250 mg·kg^(-1)蒸大蒜多糖进行治疗,连续干预7 d,每天1次。以稀便率、稀便级、腹泻指数、小肠推进率、苏木素-伊红(HE)染色为指标考察蒸大蒜多糖改善幼年大鼠腹泻症状的作用。收集大鼠的粪便,进行16S rRNA高通量测序并分析其结果。结果:与模型组比较,西药组与蒸大蒜多糖高、低剂量组幼年大鼠一般情况明显好转,平均稀便率及腹泻指数显著下调(P<0.01),小肠推进率明显降低(P<0.05),HE染色显示蒸大蒜多糖治疗之后,结肠组织炎性细胞浸润改善,肠腺组织结构恢复趋于正常,其他结构无异常,说明蒸大蒜多糖能显著改善幼年大鼠的腹泻状况。菌群测序结果可知,蒸大蒜多糖对幼年菌群失调性腹泻模型大鼠菌群丰度有调节作用,提高幼年菌群失调性腹泻模型大鼠菌群生物丰富度指数与多样性指数,其中拟杆菌门(Bacteroidota)丰度增加,厚壁菌门(Firmicutes)与变形菌门(Proteobacteria)丰度降低。结论:蒸大蒜多糖能够通过调节菌群丰度,从而起到治疗幼年大鼠菌群失调性腹泻的作用。Objective:To verify the efficacy of steamed Allii Sativi Bulbus polysaccharide in regulating the intestinal flora of young dysbiosis-induced diarrhea rats based on 16S rRNA gene sequencing.Method:The young SD rats were randomly divided into blank group,model group,positive drug group(bifid triple viable capsules),and high-dose and low-dose steamed Allii Sativi Bulbus polysaccharide groups,six in each group.The dysbiosis-induced diarrhea rat model was established,and the blank group and the model group were given normal saline by gavage(each 10 mL·kg^(-1)),and the high-dose and low-dose steamed Allii Sativi Bulbus polysaccharide groups were administered with corresponding drugs(500 mg·kg^(-1)and 250 mg·kg^(-1),respectively),once a day for seven consecutive days.The loose stool rate,loose stool grade,diarrhea index,small intestine propulsion rate and hematoxylin-eosin(HE)staining were used as indexes to investigate the effect of steamed Allii Sativi Bulbus polysaccharide on improving diarrhea symptoms in young rats.The feces of rats were collected for 16S rRNA gene high-throughput sequencing.Result:Compared with the model group,the positive drug group and the high-dose and low-dose steamed Allii Sativi Bulbus polysaccharide groups had alleviated symptoms,down-regulated loose stool rate and diarrhea index(P<0.01)and decreased small intestine advancement rate(P<0.05).HE staining showed that after the treatment with steamed Allii Sativi Bulbus polysaccharide,the inflammatory cell infiltration of the colon tissue was improved and the intestinal gland recovered to the normal condition,which indicated that steamed Allii Sativi Bulbus polysaccharide could significantly ameliorate the diarrhea in young rats.The sequencing results revealed that steamed Allii Sativi Bulbus polysaccharide had a moderating effect on the abundance of the intestinal flora of young dysbiosisinduced diarrhea rats,elevating the flora richness and diversity indexes.Specifically,the abundance of Bacteroidota was increased while that of Firmicutes

关 键 词：大蒜多糖 蒸制 腹泻 药效 机制 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学] R22R2-031R33