方格星虫三肽对黑色素瘤A375细胞的增殖抑制作用  

Inhibition effect of tripeptide of Sipunculus nudus Linnaeus on proliferation of melanoma A375 cells

作　　者：戚怡 陈起周 王艺瑾 叶子秋 吴科锋 叶华 罗辉 欧阳茜茜 QI Yi;CHEN Qi-zhou;WANG Yi-jin;YE Zi-qiu;WU Ke-feng;YE Hua;LUO Hui;OUYANG Qian-qian(Marine Biomedical Research Institution,Guangdong Medical University,Zhanjiang,524023,China;Guangdong Provincial Laboratory of Southern Marine Science and Engineering,Zhanjiang 524023,China;Zhanjiang Marine Biomedical Research Institute of Guangdong,Zhanjiang 524023,China)

机构地区：[1]广东医科大学海洋医药研究院,广东湛江524000 [2]南方海洋科学与工程广东省实验室(湛江),广东湛江524000 [3]广东湛江海洋医药研究院,广东湛江524000

出　　处：《广东医科大学学报》2022年第3期267-271,共5页Journal of Guangdong Medical University

基　　金：广东医科大学博士科研启动基金(GDMUB2021005);广东医科大学海洋医药研究院南海海洋生物医药资源研发公共服务平台;南方海洋科学与工程广东省实验室(湛江)基金(ZJW-2019-007)。

摘　　要：目的 观察方格星虫三肽对人黑色素瘤细胞A375细胞增殖的影响。方法 CCK-8法观察方格星虫三肽对A375细胞生长的影响;平板克隆实验观察其对A375细胞克隆形成能力的影响;transwell细胞迁移实验检验其对A375细胞迁移的影响;活性氧(ROS)、乳酸脱氢酶(LDH)实验检测其对A375细胞ROS和LDH含量的影响。结果 方格星虫三肽可抑制A375细胞增殖、克隆形成、细胞迁移、LDH释放,增加ROS含量(P<0.05或0.01)。结论 方格星虫三肽对A375细胞增殖有明显抑制作用。Objective To investigate the effect of Sipunculus nudus Linnaeus-derived tripeptide on proliferation of human melanoma A375 cells. Methods The proliferation, clonality, migration, and reactive oxygen species(ROS) and lactate dehydrogenase(LDH) levels in A375 cells were respectively detected by CCK-8, plate clone formation, transwell, DCFH-DA,and ELISA after pretreatment with the tripeptide. Results The tripeptide decreased proliferation, clone formation, migration,and LDH level in A375 cells, and increased ROS content(P<0.05 or 0.01). Conclusion The tripeptide derived from Sipunculus nudus Linnaeus can significantly inhibit the proliferation of A375 cells.

关 键 词：方格星虫 活性肽 A375细胞 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]