基于谱效分析评价电子束辐照灭菌对山银花药材质量的影响  

Evaluation on the Quality of Lonicerae flos of Electron Beam Irradiation Sterilization Based on Spectrum-Effect Analysis

作　　者：徐远芳[1] 彭玲[1] 李鹏辉 张祺玲[1] 高美须[3] 周毅吉 李文革[1] 邓钢桥[1] XU Yuanfang;PENG Ling;LI Penghui;ZHANG Qiling;GAO Meixu;ZHOU Yiji;LI Wenge;DENG Gangqiao(Institute of Nuclear Agricultural Science and Space Breeding,Hunan Academy of Agriculrural Sciences/Hunan province Engineering Technology Research Center of Agricultural Biological Irradiation/Hunan Province Biological Irradiation Techonology Engineering Research Center,Changsha,Hunan 410125;Hunan Academy of Traditional Chinese Medicine,Changsha,Hunan 410006;Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193)

机构地区：[1]湖南省农业科学院核农学与航天育种研究所/湖南省农业生物辐照工程技术研究中心/生物辐照技术湖南省工程研究中心,湖南长沙410125 [2]湖南省中医药研究院,湖南长沙410006 [3]中国农业科学院农产品加工研究所,北京100193

出　　处：《核农学报》2022年第4期745-753,共9页Journal of Nuclear Agricultural Sciences

基　　金：湖南省农业科技创新资金(2019ZYC02);湖南省自然科学基金科药联合基金(2020JJ9041)。

摘　　要：为评价电子束辐照灭菌对山银花药材质量的影响,分别采用0、2.8、6.3、9.5、18.8 kGy吸收剂量的电子束辐照处理山银花及其提取物,研究不同剂量电子束辐照对其生物负载及指标成分绿原酸含量、抗氧化活性及指纹图谱的影响。结果表明,电子束辐照能够有效降低山银花及其提取物中需氧菌、霉菌和酵母菌总数,随着吸收剂量增大,其存活数显著降低;电子束辐照对山银花及其提取物中绿原酸的含量及1,1-二苯基-2-苦基肼自由基(DPPH·)清除率的影响不大,其变化与吸收剂量均无显著线性相关性(P>0.05),当吸收剂量达到18.8 kGy时,与未辐照样品相比均无显著差异(P>0.05)。不同吸收剂量电子束辐照处理的山银花及其提取物指纹图谱的相似度均为1;聚类分析(CA)结果也表明经不同吸收剂量电子束辐照处理的样品与未辐照样品未呈现显著差异。综上,电子束辐照灭菌对中药材山银花及其提取物的质量无明显影响。本研究利用谱效分析,系统评价电子束辐照灭菌对中药材山银花及其提取物质量的影响,为电子束辐照技术在山银花药材贮藏中的科学应用提供了参考依据。In order to evaluate the effects of electron beam irradiation sterilization on the quality of Chinese medicinal materials Lonicerae flos.The sterilization effect,the content of the index components chlorogenic acid,antioxidant activity and fingerprint of Lonicerae flos and its extract were evaluated when they were irradiated by electron beam with the absorbed doses of 0,2.8,6.3,9.5,18.8 kGy.The results showed that electron beam irradiation could effectively reduce the number of total aerobic bacteria count,total yeast and mold count in Lonicerae flos and its extract,and the survival decreased obviously with the increase of absorbed dose.The content of chlorogenic acid and the scavenging rate of 1,1-diphenyl-2-picrylhydrazyl(DPPH·)were not significantly affected by electron beam irradiation,and there was no obvious linear correlation with the absorbed dose(P>0.05).When the absorbed dose was increased to 18.8 kGy,the electron beam irradiation showed no significant effect on the unirradiated samples(P>0.05).The similarity of fingerprints of Lonicerae flos and its extracts treated with different absorbed doses of electron beam irradiation was 1.Cluster analysis showed that the electron beam irradiation did not make significant difference in their fingerprints.Therefore,electron beam irradiation sterilization would not significantly affect the quality of Lonicerae flos and its extract.This study can provide a reference for the scientifically application of electron beam irradiation technology in the storage of Lonicerae flos.

关 键 词：山银花药材 电子束辐照灭菌 绿原酸 抗氧化活性 指纹图谱 

分 类 号：R284[医药卫生—中药学]