新型甜味剂阿洛酮糖绿色生物制造工艺研究进展  

Research Progress on Green Bio-Manufacturing Technology of New Sweetener D-Psicose

作　　者：王琪 彭超 周卫强 唐堂 何太波 裴成利 梁颖超 武丽达 李凡 李义 佟毅[4,5] WANG Qi;PENG Chao;ZHOU WeiQiang;TANG Tang;HE Taibo;PEI Chengli;LIANG Yingchao;WU Lida;LI Fan;LI Yi;TONG Yi(COFCO Nutrition and Health Research Institute Co.,Ltd.,Beijing 102209,China;Nutrition Health and Food Safety Beijing Key Laboratory,Beijing 102209,China;Beijing Engineering Laboratory for Geriatric Nutrition Food Research,Beijing 102209,China;COFCO Biotechnology Co.,Ltd.,Bengbu 233010,China;National Engineering Research Center of Corn Deep Processing,Changchun 130033,China)

机构地区：[1]中粮营养健康研究院有限公司,北京102209 [2]营养健康与食品安全北京市重点实验室,北京102209 [3]老年营养食品研究北京市工程实验室,北京102209 [4]中粮生物科技股份有限公司,安徽蚌埠233010 [5]玉米深加工国家工程研究中心,吉林长春130033

出　　处：《生物化工》2022年第3期170-176,共7页Biological Chemical Engineering

摘　　要：D-阿洛酮糖是一类热量较低但甜度很高的新型功能性甜味剂,具有抗氧化、降血糖和降血脂等功效。本文综述了D-阿洛酮糖的生理功能和机制、生产阿洛酮糖关键酶的来源和性质,阐述了生物法合成D-阿洛酮糖的工艺方法的基本过程,探讨了阿洛酮糖差向异构酶生产工程菌株的表达体系和D-阿洛酮糖的分离纯化方法及结晶工艺。D-psicose is a new type of functional sweetener with low calorie but high sweetness,which has antioxidant,hypoglycemic and hypolipidemic effects.This paper reviews the physiological functions and mechanisms of D-psicose,the sources and properties of key enzymes in the production of D-psicose.The basic process of the Biotechnology synthesis of D-psicose is described,the expression system of the engineering strain producing psicose epimerase,the Methods of separation and purification of D-psicose and the crystallization process were discussed.

关 键 词：D-阿洛酮糖 D-阿洛酮糖3-差向异构酶 菌株表达系统 分离纯化 

分 类 号：TS264.9[轻工技术与工程—发酵工程]