江苏省2016—2019年不同来源小肠结肠炎耶尔森菌血清、生物型分布及分子特征分析  

Analysis serum,biotype distribution and molecular characteristics of Yersinia enterocolitic from different hosts in Jiangsu Province,2016-2019

作　　者：周璐[1] 段然[2] 祝雯雯[3] 朱莹莹 鲍倡俊[1] 王鑫[2] 谈忠鸣[1] ZHOU Lu;DUAN Ran;ZHU Wenwen;ZHU Yingying;BAO Changjun;WANG Xin;TAN Zhongming(NHC Key Laboratory of Enteric Pathogenic Microbiology,Jiangsu Provincial Centre for Disease Control and Prevention,Nanjing 210009;National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206;Xuzhou Centre for Disease Control and Prevention,Xuzhou 221002;Dongtai Centre for Disease Control and Prevention,Yancheng 224299,China)

机构地区：[1]国家卫生健康委员会肠道病原微生物重点实验室,江苏省疾病预防控制中心,江苏南京210009 [2]中国疾病预防控制中心传染病预防控制所,北京102206 [3]徐州市疾病预防控制中心,江苏徐州221002 [4]东台市疾病预防控制中心,江苏东台224299

出　　处：《南京医科大学学报（自然科学版）》2022年第5期724-728,745,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家科技重大专项(2018ZX10713-003-002,2018ZX10713-001-002);江苏省卫生健康委科研重点项目(ZD2021037)。

摘　　要：目的:了解江苏地区2016—2019年间不同来源小肠结肠炎耶尔森菌血清、生物型分布及分子特征。方法:收集2016—2019年江苏地区各类标本进行小肠结肠炎耶尔森菌分离培养,对分离株进行生化和血清分型,并通过二代测序方法进行毒力基因和多位点序列分析。结果:2016—2019年在江苏地区共采集各类标本6398份,分离小肠结肠炎耶尔森菌186株,其中105株菌为血清型无法鉴定,可鉴定血清型中以O:8、O:3为主,存在3个生物型,大部分为生物型1A。通过测序分析发现毒力基因以Ⅲ型(ail-、ystA-、ystB+、yadA-、virF-)和Ⅳ型(ail-、ystA-、ystB-、yadA-、virF-)为主,多位点序列分析(MLST)将所有分离株分为81个ST型。结论:江苏地区不同来源小肠结肠炎耶尔森菌血清型较复杂,生物型相对集中,腹泻患者中可分离到毒力基因Ⅳ型菌株,ST基因型呈多样性发散分布,应重点加强猪、鸡、狗等动物的管控和监测。Objective:To reveal the serum,biotype distribution and molecular characteristics of isolates from different hosts in Jiangsu Province,2016-2019.Methods:Different kinds of specimens were collected for isolation strains,the isolated strains of Yersinia enterocolitic were evaluated by serotyping and biotyping.The five virulence genes(ail,virF,yadA,ystA and ystB)and multilocus sequence typing were analysed by using next-generation sequencing technology.Results:In this study,6398 specimens were collected,and 186 strains of Yersinia enterocolitic were isolated.The serotype of 109 strains could not be identified,the dominant serotype that can be identified are O:8 and O:3.There are 3 biotypes of all isolates,most of which are biotype 1A.Based on sequencing analysis,typeⅢand typeⅣare the mainly virulence gene type in this area.All strains were divided into 81 STs by MLST.Conclusion:The serotype of Yersinia enterocolitic isolated from different hosts are complex,the biotype is relatively concentrated.Virulence gene typeⅣstrains can be isolated from diarrhea patients.ST genotypes are distributed divergently.The hosts of pig,chick and dog should be supervision and surveillance.

关 键 词：小肠结肠炎耶尔森菌 血清型 全基因组测序 毒力基因 多位点序列分析 

分 类 号：R181.32[医药卫生—流行病学]