Linear polyketides produced by co-culture of Penicillium crustosum and Penicillium fellutanum  

作　　者：Guihong Yu Peng Sun Reyilamu Aierken Chunxiao Sun Zhenzhen Zhang Qian Che Guojian Zhang Tianjiao Zhu Qianqun Gu Mingyu Li Dehai Li 

机构地区：[1]Key Laboratory of Marine Drugs,Chinese Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao,266003,China [2]Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao,266237,China [3]Fujian Provincial Key Laboratory of Innovative Drug Target Research,School of Pharmaceutical Science,Xiamen University,Xiamen,361102,China [4]School of Life Sciences,Shandong Province Key Laboratory of Applied Mycology,Qingdao Agricultural University,Qingdao,266109,China

出　　处：《Marine Life Science & Technology》2022年第2期237-244,共8页海洋生命科学与技术（英文）

基　　金：supported by the National Natural Science Foundation of China(41806167,81670709);Project funded by China Postdoctoral Science Foundation(2017M622286);Qingdao Postdoctoral Applied Research Project Financially Supported by Qingdao Municipal Bureau of Human Resource and Social Security,Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology(LMDBKF201805);High-level Talents Research Fund of Qingdao Agricultural University(Grants 665/1120034);We thank Dr.Zhiyuan Gong(National Univeristy of Singapore)for the gift of Tg(Fabp10-rtTA:TRE-eGFPKRASV12)transgenic line.

摘　　要：Two new polyketides, penifellutins A (1) and B (2), possessing a 22 carbon linear skeleton, were isolated from a co-culture of the deep-sea-derived fungi Penicillium crustosum PRB-2 and Penicillium fellutanum HDN14-323. Meanwhile, two esterification products of 1, penifellutins C (3) and D (4), were obtained because compound 1 could be esterified spontaneously when stored in methanol. Their configurations were difficult to determine because of chiral central crowdedness, structural flexibility and instability. As such, we solved this issue by comprehensively using Mo2(OAc)4-based CD experiments, density functional theory calculation of 13C NMR, DP4 + probability analysis and many chemical reactions, including making acetonide derivative, Mosher’s method, PGME method, etc. Compounds 1 and 2 show obvious inhibitory activity on the liver hyperplasia of zebrafish larvae at a concentration of 10 μmol/L, while 3 and 4 show no activity, indicating that two carboxyls in the structure are important active sites.

关 键 词：CO-CULTURE POLYKETIDES Extraction and isolation Structure elucidation ANTIPROLIFERATION 

分 类 号：Q93[生物学—微生物学]