刺玫果籽油索氏法提取工艺优化及其体外抗氧化活性分析  

Process Optimization on Soxhlet Extraction for Seed Oil from Rosa davurica and Antioxidant Activities Analysis of Extracted Oil in Vitro

作　　者：于晓瑾[1] 刘采艳 张萌[1] 王硕 袁旭 韩华[1] YU Xiao-jin;LIU Cai-yan;ZHANG Meng;WANG Shuo;YUAN Xu;HAN Hua(College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国食物与营养》2022年第6期31-38,共8页Food and Nutrition in China

基　　金：国家自然科学基金项目(项目编号:81973588);哈尔滨市应用技术开发项目(项目编号:2016RQQXJ133)。

摘　　要：目的:对刺玫籽油进行体外抗氧化活性评价,旨在为刺玫籽油的进一步研究及刺玫果的综合开发利用提供科学依据。方法:以刺玫果籽为原料,利用索氏提取法提取刺玫果籽油,并以刺玫果籽油的提取率为指标,在单因素分析的基础上,选取提取温度、粉碎目数、溶剂体积3个因素进行响应面优化试验。以维生素C为阳性对照,对所提取的刺玫果籽油进行体外抗氧化活性分析。结果:当溶剂体积333 mL、提取温度82℃、粉碎度60目时,刺玫果籽油的提取率为18.84%。此外,刺玫果籽油清除ABTS自由基、DPPH自由基、超氧阴离子自由基的作用存在量效关系,且IC_(50)值分别为29.73、34.58、40.54μg/mL,而同浓度维生素C清除自由基的IC_(50)值分别为26.51、49.62、26.37μg/mL,此外,刺玫果籽油还具有一定的还原力。结论:利用响应面优化法优化提取工艺参数可提高刺玫籽油的提取率,刺玫籽油还具有较好的抗氧化活性,可作为一种潜在可用的天然抗氧化剂。[Objective]The antioxidant properties of Rosa davurica seed oil in vitro was evaluated to provide scientific basis for further studying and comprehensive development and utilization for Rosa davurica.MethodRosa davurica seed oil was extracted by Soxhlet Extraction.With extract yields as target,based on the analysis of single factors such as extraction temperature,crushing mesh number,extraction time and solvent volume.The experiment of response surface optimization was selected for extraction temperature,crushing mesh number,extraction time,solvent volume as variables,as well as the in vitro antioxidant activity of Rosa davurica seed oil was analyzed using vitamin C as positive control.ResultThe optimum extraction conditions were projected as solvent volume 333 mL,extraction temperature 82℃,crushing mesh number 60 mesh,and the yield of 18.84%were obtained.Moreover,these results demonstrated that Rosa davurica seed oil had dose-effect on the scavenging rate of ABTS,DPPH,superoxide anions that the IC50 values were 29.73μg/mL,34.58μg/mL,40.54μg/mL,respectively,compared to that of vitamin C group was 26.51μg/mL,49.62μg/mL,26.37μg/mL,respectively.Besides,it also possessed definite reducing powering.Conclusion Response Surface Optimization Method is adopted to improve the extraction rate of Rosa davurica seed oil,the seed oil from Rosa davurica shows significant antioxidant capacity,and can be usedas a potentially available natural antioxidant.

关 键 词：刺玫果 种子油 索氏提取法 中心组合响应面设计 抗氧化活性 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]