Dl-3-n butylphthalide attenuates brain injury caused by cortical infarction accompanied by cranial venous drainage disturbance  

作　　者：Kangping Song Xiuli Zeng Xiaomei Xie Rongxuan Zhu Jianye Liang Guobing Chen Li’an Huang 

机构地区：[1]Department of Neurology,Jinan University First Affiliated Hospital,Guangzhou,China [2]Medical Imaging Center,Jinan University First Affiliated Hospital,Guangzhou,China [3]Institute of Geriatric Immunology,Medical college of Jinan University,Guangzhou,China

出　　处：《Stroke & Vascular Neurology》2022年第3期222-236,共15页卒中与血管神经病学（英文）

基　　金：This study was funded by the National Natural Science Foundation of China(No.81971120);the Science and Technology Planning Project of Guangdong Province of China(No 2017A02021520081971120).

摘　　要：Background Cerebral venous disorder may have a harmful effect on ischaemic stroke;however,the underlying mechanism remains to be elucidated.Although Dl-3-n butylphthalide is a multitarget agent for antiischaemic stroke,its neuroprotective role in brain ischaemia accompanied by brain venous disturbance remains unclear.In this study,we induced cerebral venous disturbance by the occlusion of bilateral external jugular veins(EJVs)to explore the potential mechanism of the adverse effects of cerebrovenous disorders in cerebral infarction and explore the protective effect of Dl-3-n butylphthalide on cerebral infarction accompanied through cerebral venous disturbance.Methods Cerebral venous disturbance was induced in Sprague-Dawley rats through the permanent occlusion of bilateral EJVs,and cerebral ischaemic stroke was induced through the permanent occlusion of the right cortical branches of the middle cerebral artery.2,3,5-triphenyltetrazolium chloride staining,MRI,Evans blue extravasation and behavioural test were performed to evaluate infarction volume,cerebral blood flow(CBF),blood-brain barrier(BBB)integrity and neurological function.Immunofluorescence staining and western blot analysis were performed to detect loss of neuron,endothelial cells,pericytes and tight junctions.Results Bilateral EJVs occlusion did not cause cerebral infarction;however,it increased the infarction volume compared with the simple middle cerebral artery occlusion(MCAO)group,accompanied by severe neuron loss,worse neurological function,lower CBF,increased EJVs pressure,exacerbated Evans blue extravasation and brain oedema,as well as attenuated angiogenesis.Dl-3-n butylphthalide displayed a neuroprotective effect in rats with MCAO accompanied by EJVs occlusion by reducing neuron loss,accelerating CBF restoration,promoting angiogenesis and relieving BBB damage.Conclusion Bilateral EJVs occlusion did not significantly affect normal rats but aggravated brain damage in the case of ischaemic stroke.Dl-3-n butylphthalide treatment plays a neuroprote

关 键 词：protective INFARCTION DISTURBANCE 

分 类 号：R741[医药卫生—神经病学与精神病学]