GABA受体拮抗剂荷包牡丹碱和CGP52432对厚壳贻贝幼虫附着变态的影响  

Effects of GABA receptor antagonists bicuculline and CGP52432 on larval settlement and metamorphosis of the mussel(Mytilus coruscus)

作　　者：王林雨 史雪 杨跃明 王雨晴 梁箫[1,2] 杨金龙[1,2,3] 李一峰 WANG Linyu;SHI Xue;YANG Yueming;WANG Yuqing;LIANG Xiao;YANG Jinlong;LI Yifeng(International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory,Guangzhou 511458,China)

机构地区：[1]上海海洋大学,国家海洋生物科学国际联合研究中心,上海201306 [2]上海海洋大学,水产种质资源发掘与利用教育部重点实验室,上海201306 [3]南方海洋科学与工程广东省实验室,广东广州511458

出　　处：《水产学报》2022年第6期942-949,共8页Journal of Fisheries of China

基　　金：国家自然科学基金(32172992);国家重点研发计划(2018YFD0900601);上海市优秀学术带头人计划(20XD1421800);南方海洋科学与工程广东省实验室人才团队引进重大专项(GML2019ZD0402)。

摘　　要：为研究GABA及其受体拮抗剂荷包牡丹碱(GABA_(A)受体)和CGP52432(GABA_(B)受体)在厚壳贻贝幼虫附着变态中的调控作用,实验通过分析厚壳贻贝不同发育阶段幼虫转录组数据获得GABA受体相关蛋白基因(GABARAP)和GABA_(B2)受体基因(GABA_(B2)R),发现GABA_(B2)R在变态前期的眼点幼虫阶段相较于其他阶段幼体显著高表达。通过实时荧光定量PCR验证也得到相似的表达模式,显示GABA_(B2)R可能参与调控厚壳贻贝幼虫变态发育。药理学实验结果显示,10^(−4)mol/L GABA对厚壳贻贝幼虫的变态具有诱导活性(27.2%±3.0%)。在拮抗剂实验中,10^(−6)~10^(−4)mol/L荷包牡丹碱和CGP52432均显著抑制了厚壳贻贝幼虫变态,且抑制作用随浓度升高而增强。实验还发现GABA及其受体拮抗剂都抑制了厚壳贻贝幼虫的游泳行为。研究表明,GABA_(A)或GABA_(B)受体拮抗剂均对厚壳贻贝幼虫附着变态具有抑制作用,GABA受体可能参与调控厚壳贻贝幼虫附着变态过程。γ-aminobutyric acid(GABA)is an important inhibitory neurotransmitter in the central nervous system in mammals.In marine invertebrates,GABA has been reported to induce larval settlement and metamorphosis in many species.The pharmacological experiment was conducted to study the effects of GABA and its receptor inhibitors Bicuculline(GABA_(A)receptor)and CGP52432(GABA_(B)receptor)on larval settlement and metamorphosis of M.coruscus.GABA receptor-associated protein(GABARAP)and GABA_(B2)receptor(GABA_(B2)R)genes have been found in the transcriptome data of different developmental stages of M.coruscus.GABA_(B2)R was significantly highly expressed in the pediveliger stage than in other development stages.A similar expression pattern was also observed using the real-time fluorescent quantitative PCR experiment,suggesting that GABA_(B2)R may be involved in the larval metamorphosis.The results showed that 10^(−4)mol/L GABA induced 27.2%±3.0%of the pediveliger larvae metamorphosis.Bicuculline and CGP52432 significantly inhibited larval metamorphosis compared to the control larvae.The higher concentrations of antagonists showed more pronounced effects than lower concentrations.In addition,both GABA and its receptor antagonists inhibited the swimming behavior of mussel larvae.Both GABA_(A)and GABA_(B)receptor antagonists inhibited the larval settlement and metamorphosis of M.coruscus,suggesting GABA receptors may be involved in mediating larval settlement and metamorphosis of M.coruscus.The present study contributes to further exploring the mechanism of the GABAergic signaling system regulating larval settlement and metamorphosis of M.coruscus.

关 键 词：厚壳贻贝 GABA受体 幼虫 附着变态 

分 类 号：S917.4[农业科学—水产科学]