川芎-葛根药对及其单味药挥发性成分GC-MS分析  

GC-MS analysis of volatile components in Chuanxiong-Gegen pair and its single herb

作　　者：李书帆 江汉美[1] 刘毅[1] 刘天琪 LI Shu-fan;JIANG Han-mei;LIU Yi;LIU Tian-qi(Hubei University of Chinese Medicine/Hubei Medical Plant Research and Development Center,Wuhan 430065,China)

机构地区：[1]湖北中医药大学/湖北省药用植物研发中心,武汉430065

出　　处：《湖北农业科学》2022年第11期123-127,138,共6页Hubei Agricultural Sciences

摘　　要：分析了川芎-葛根药对与单味药挥发性成分的组成和药理作用。采用固相微萃取-气相色谱-质谱联用技术,对川芎-葛根药对与单味药中挥发性化学成分进行定性和定量分析,以峰面积归一化法计算各组分的相对含量。结果初步鉴定出68种化合物。药对、川芎和葛根中含量最高的挥发性成分(相对含量)依次为β-甲基苯丙醛(46.84%)、间甲酚(48.49%)、(-)-α-荜澄茄油烯(13.85%)。组成药对后新增21种成分,原有组分减少32种。证明该方法稳定可靠,适用于川芎-葛根药对及其单味药挥发性成分的快速分析,可为川芎、葛根的临床应用提供一定的科学依据,二者联用分析药对与单味药的挥发性成分能够得到更加全面的信息,新增成分对后续川芎-葛根药对及其复方制剂的相关研究具有指导作用,药对在配伍前后成分发生较为明显的变化。The composition and pharmacological effects of volatile components in Chuanxiong-Gegen pair and single herb were analyzed. The volatile components in Chuanxiong-Gegen pair and single herb were qualitatively and quantitatively analyzed by solid phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS),and the relative contents of each component were calculated by peak area normalization method. The result was a preliminary identification of 68 compounds. The highest volatile components(relative content)were β-methylphenylpropanal(46.84%),m-cresol(48.49%)and(-)-α-solanolene(13.85%). After the composition of drug pairs,21 new components were added,and 32 original components were reduced. It was proved that this method was stable and reliable,and it was suitable for the rapid analysis of volatile components of Chuanxiong-Gegen pair and its single herb,which could provide a scientific basis for the clinical application of Chuanxiong and Gegen. The combined analysis of volatile components in Chuanxiong-Gegen pair and single herb could obtain more comprehensive information. The newly added components could guide the subsequent research on Chuanxiong-Gegen pair and its compound preparations,and the components in Chuanxiong-Gegen pair changed significantly before and after compatibility.

关 键 词：川芎-葛根药对 挥发性成分 顶空固相微萃取 气质联用分析 

分 类 号：R284[医药卫生—中药学] R917[医药卫生—中医学]